2 Matching Annotations
  1. Jul 2018
  2. europepmc.org europepmc.org
    https://www.ncbi.nlm.nih.gov/pubmed/25819921
    1
    1. PubMedCommonsArchive 02 Jul 2018

      On 2016 Jun 20, Darya Vanichkina commented:

      UCSC genome browser in-silico PCR on hg19 UCSC genes predicts that a different lincRNA (on a different chromosome; chr10:102,133,333-102,148,116 vs chr21) will be amplified using the primers reported to target linc00320. How was this off-target PCR controlled for? Was the PCR product sequenced to confirm correct template was being amplified?

      >uc001kra.4__LINC00263:555+727 173bp GACTCCTTTGGGAGACCAGTG AGGTCACAGGGGATTTGATGG GACTCCTTTGGGAGACCAGTGccctgttgtcgccctcactccgtgaggag atccacctatgatctcaggtcctcagaccaaccagcccaaggaacatctt gccaatttcaaatcggaagataggagtgtcaggcctctgagtccaagcta agCCATCAAATCCCCTGTGACCT

      >uc001kqz.4__LINC00263:555+724 170bp GACTCCTTTGGGAGACCAGTG AGGTCACAGGGGATTTGATGG GACTCCTTTGGGAGACCAGTGccctgttgtcgccctcactccgtgaggag atccacctatgatctcaggtcctcagaccaaccagcccaaggaacatctt gccaatttcaaatcggataggagtgtcaggcctctgagtccaagctaagC CATCAAATCCCCTGTGACCT

      This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY.
      PubMedCommonsArchive PMID:25819921
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    europepmc.org/abstract/MED/25819921
  3. Feb 2018
  4. europepmc.org europepmc.org
    https://www.ncbi.nlm.nih.gov/pubmed/25819921
    1
    1. PubMedCommonsArchive 09 Feb 2018

      On 2016 Jun 20, Darya Vanichkina commented:

      UCSC genome browser in-silico PCR on hg19 UCSC genes predicts that a different lincRNA (on a different chromosome; chr10:102,133,333-102,148,116 vs chr21) will be amplified using the primers reported to target linc00320. How was this off-target PCR controlled for? Was the PCR product sequenced to confirm correct template was being amplified?

      >uc001kra.4__LINC00263:555+727 173bp GACTCCTTTGGGAGACCAGTG AGGTCACAGGGGATTTGATGG GACTCCTTTGGGAGACCAGTGccctgttgtcgccctcactccgtgaggag atccacctatgatctcaggtcctcagaccaaccagcccaaggaacatctt gccaatttcaaatcggaagataggagtgtcaggcctctgagtccaagcta agCCATCAAATCCCCTGTGACCT

      >uc001kqz.4__LINC00263:555+724 170bp GACTCCTTTGGGAGACCAGTG AGGTCACAGGGGATTTGATGG GACTCCTTTGGGAGACCAGTGccctgttgtcgccctcactccgtgaggag atccacctatgatctcaggtcctcagaccaaccagcccaaggaacatctt gccaatttcaaatcggataggagtgtcaggcctctgagtccaagctaagC CATCAAATCCCCTGTGACCT

      This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY.
      PubMedCommonsArchive PMID:25819921
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    europepmc.org/abstract/MED/25819921