On 2015 Dec 07, Sanjay Kumar commented:

We appreciate the interest in our work. While we are unable to comment on the internalization mechanisms of the SmartFlare reagents based on our data, our independent RT-PCR measurements (Fig. 3) are consistent with the SmartFlare data and confirm that matrix stiffness and ligand density regulate miR18a levels in this system.

On 2015 Nov 18, Raphael Levy commented:

This article uses the SmartFlare technology to detect miR18a. I would be most interested in the authors' opinion on how these probes escape endosomes to report on miR18a level. In our experience, they do not and fluorescence increase is most likely correlated with degradation in endosomes. It might be quite plausible that endocytosis "is non-linearly regulated by matrix stiffness and fibronectin density in glioma cells."

On 2015 Nov 18, Raphael Levy commented:

This article uses the SmartFlare technology to detect miR18a. I would be most interested in the authors' opinion on how these probes escape endosomes to report on miR18a level. In our experience, they do not and fluorescence increase is most likely correlated with degradation in endosomes. It might be quite plausible that endocytosis "is non-linearly regulated by matrix stiffness and fibronectin density in glioma cells."

On 2015 Dec 07, Sanjay Kumar commented:

We appreciate the interest in our work. While we are unable to comment on the internalization mechanisms of the SmartFlare reagents based on our data, our independent RT-PCR measurements (Fig. 3) are consistent with the SmartFlare data and confirm that matrix stiffness and ligand density regulate miR18a levels in this system.