On 2015 Oct 29, Dmitri Graifer commented:

I have read with great interest this excellent paper. Regretfully, I found that the authors cite earlier studies in somewhat careless way. First, when mentioning biochemical data on eRF1 motifs involved in stop codon recognition, they ignore data obtained by site-directed cross-linking, which for the first time directly demonstrated that one of the mentioned motifs, GTS, is actually located close to the purines of the A site bound stop codon in the mammalian ribosomal termination complexes (Bulygin et al., 2010, RNA). In this regard, the authors cite only the later paper by Wong et al. (Nucleic Acids Res., 2012) as ref. 25, where a suggestion on implication of this motif in stop codon recognition has been made based on the application of a model ribosome-free system but this paper does not contain any original evidence for the proximity of GTS motif to the stop codon bound at the ribosomal A site. Besides, I have found an “empty” reference 52 (Blanchet et al., Nucleic Acids Res., 2015), which does not contain information on contacts of Arg residues in eRF1 positions 65 and 68 with rRNA as one could expect from the phrase “…and the following Arg65 and Arg68 interact with ribosomal RNA (52)”. In particular, in the paper by Blanchet et al., 2015 (ref. 52) Arg68 is not mentioned at all, and interaction of Arg65 with the rRNA is only hypothesized in the middle part of the penultimate paragraph of the Discussion but it is not shown. I guess that inappropriate referencing corrupts really existing priority in the field and gives distorted ideas on the actually obtained scientific results.

On 2015 Oct 29, Dmitri Graifer commented:

I have read with great interest this excellent paper. Regretfully, I found that the authors cite earlier studies in somewhat careless way. First, when mentioning biochemical data on eRF1 motifs involved in stop codon recognition, they ignore data obtained by site-directed cross-linking, which for the first time directly demonstrated that one of the mentioned motifs, GTS, is actually located close to the purines of the A site bound stop codon in the mammalian ribosomal termination complexes (Bulygin et al., 2010, RNA). In this regard, the authors cite only the later paper by Wong et al. (Nucleic Acids Res., 2012) as ref. 25, where a suggestion on implication of this motif in stop codon recognition has been made based on the application of a model ribosome-free system but this paper does not contain any original evidence for the proximity of GTS motif to the stop codon bound at the ribosomal A site. Besides, I have found an “empty” reference 52 (Blanchet et al., Nucleic Acids Res., 2015), which does not contain information on contacts of Arg residues in eRF1 positions 65 and 68 with rRNA as one could expect from the phrase “…and the following Arg65 and Arg68 interact with ribosomal RNA (52)”. In particular, in the paper by Blanchet et al., 2015 (ref. 52) Arg68 is not mentioned at all, and interaction of Arg65 with the rRNA is only hypothesized in the middle part of the penultimate paragraph of the Discussion but it is not shown. I guess that inappropriate referencing corrupts really existing priority in the field and gives distorted ideas on the actually obtained scientific results.