On 2015 Nov 02, George McNamara commented:

Could the authors please add scale bars to their light microscope image data?

With respect to figure 3, "dendrites of the NK cells penetrating into the target cells", the word penetrating implies piercing the target cell plasma membrane. SEM cannot show this. Even multicolor fluorescence microscopy would not show this. I suggest Transmission electron microscopy and am surprised the reviewer did not ask for this and the authors did not volunteer it.

Morphology and mechanism by which real Natural Killer cells kill is by immune synapse formation and directed secretion of perforin and granzymes -- not "piercing" target cells with sword like filopodia. As the authors point out in their discussion, the induced cell type may not be either a dendritic cell or a natural killer cell, so these "AMLiK" (my suggested acronym) do not need to follow normal cell's playbooks.

On 2015 Nov 02, George McNamara commented:

Could the authors please add scale bars to their light microscope image data?

With respect to figure 3, "dendrites of the NK cells penetrating into the target cells", the word penetrating implies piercing the target cell plasma membrane. SEM cannot show this. Even multicolor fluorescence microscopy would not show this. I suggest Transmission electron microscopy and am surprised the reviewer did not ask for this and the authors did not volunteer it.

Morphology and mechanism by which real Natural Killer cells kill is by immune synapse formation and directed secretion of perforin and granzymes -- not "piercing" target cells with sword like filopodia. As the authors point out in their discussion, the induced cell type may not be either a dendritic cell or a natural killer cell, so these "AMLiK" (my suggested acronym) do not need to follow normal cell's playbooks.