On 2016 Sep 12, Massimiliano Tognolini commented:

2,5-dimethylpyrrolyl benzoic acid (compound 1) is a promiscuous and reactive chemical substance able to interfere with a multitude of biological targets. MOreover: - “… newly synthesized compound 1 did not show detectable inhibition of ephrin—A5 binding to EphA4 in ELISAs or EphA2 phosphorylation in cells stimulated with ephrin-A1…” when tested immediately after its chemical synthesis as white and crystalline powder. Moreover, the same Authors further explained these findings stating that “…when left exposed to air at room temperature in dry form, compound 1 acquired a progressively darker brown color. Concomitantly, the compound became progressively more active in ELISAs measuring the inhibition of ephrin-A5-EphA4 binding” (Noberini R et al Chem. Biol. Drug Des.2011; 78, 667–678). - Compound 1, spontaneously forms a mixture of reactive polymers, with an average molecular weight of 40kDa, likely responsible for the aspecific effect of compound (Zhu W et al. Chem. - Eur. J. 2013; 19, 8397–8400).

On 2016 Sep 12, Massimiliano Tognolini commented:

2,5-dimethylpyrrolyl benzoic acid (compound 1) is a promiscuous and reactive chemical substance able to interfere with a multitude of biological targets. MOreover: - “… newly synthesized compound 1 did not show detectable inhibition of ephrin—A5 binding to EphA4 in ELISAs or EphA2 phosphorylation in cells stimulated with ephrin-A1…” when tested immediately after its chemical synthesis as white and crystalline powder. Moreover, the same Authors further explained these findings stating that “…when left exposed to air at room temperature in dry form, compound 1 acquired a progressively darker brown color. Concomitantly, the compound became progressively more active in ELISAs measuring the inhibition of ephrin-A5-EphA4 binding” (Noberini R et al Chem. Biol. Drug Des.2011; 78, 667–678). - Compound 1, spontaneously forms a mixture of reactive polymers, with an average molecular weight of 40kDa, likely responsible for the aspecific effect of compound (Zhu W et al. Chem. - Eur. J. 2013; 19, 8397–8400).