- Jul 2018
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europepmc.org europepmc.org
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On 2016 Feb 29, Bhaskar Chandra Mohan Ramisetty commented:
One major issue with this work would be the choice of the strain. Although the strain is supposedly relA Plus, it was shown that relA1 mutation in this particular strain is not cured (Tsilibaris et. al, 2007, look into the material and methods section). In our own experiments, we found that same strains to be SMG negative meaning that these strains are deficient in production of ppGpp. It may be noted that the details of the strain construction were not given accurately in Engelberg-Kulka et. al., 1998. We would appreciate if these strains are verified independently for relA mutations. Since the work revolves around stress physiology, it might be imperative to validate these observations in E. coli MG1655 strain.
Our work on the above-stated problem is recently published http://www.ncbi.nlm.nih.gov/pubmed/27259116 Thank you.
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY. -
On 2016 Feb 29, Bhaskar Chandra Mohan Ramisetty commented:
None
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY. -
On 2016 Feb 29, Bhaskar Chandra Mohan Ramisetty commented:
None
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY.
-
- Feb 2018
-
europepmc.org europepmc.org
-
On 2016 Feb 29, Bhaskar Chandra Mohan Ramisetty commented:
None
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY. -
On 2016 Feb 29, Bhaskar Chandra Mohan Ramisetty commented:
None
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY. -
On 2016 Feb 29, Bhaskar Chandra Mohan Ramisetty commented:
One major issue with this work would be the choice of the strain. Although the strain is supposedly relA Plus, it was shown that relA1 mutation in this particular strain is not cured (Tsilibaris et. al, 2007, look into the material and methods section). In our own experiments, we found that same strains to be SMG negative meaning that these strains are deficient in production of ppGpp. It may be noted that the details of the strain construction were not given accurately in Engelberg-Kulka et. al., 1998. We would appreciate if these strains are verified independently for relA mutations. Since the work revolves around stress physiology, it might be imperative to validate these observations in E. coli MG1655 strain.
Our work on the above-stated problem is recently published http://www.ncbi.nlm.nih.gov/pubmed/27259116 Thank you.
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY.
-