On 2017 Feb 04, Misha Koksharov commented:

It's quite interesting that this type of cyclization doesn't prevent the necessary C-domain rotation and luciferase remains fully active.

Fig. 8 needs some corrections: 1) The pH values are swapped in the figure caption [(A) pH 5.5 and (B) pH 7.8]. 2) Probably, there is some problem with pH adjustment for pH 5.5. P. pyralis luciferase should give nearly monomodal red spectrum at pH 5.5, maybe with a faint green shoulder (Branchini BR, 2007, Oba Y, 2012, Riahi-Madvar A, 2009). Here it is only a faint red shoulder.

On 2017 Feb 04, Misha Koksharov commented:

It's quite interesting that this type of cyclization doesn't prevent the necessary C-domain rotation and luciferase remains fully active.

Fig. 8 needs some corrections: 1) The pH values are swapped in the figure caption [(A) pH 5.5 and (B) pH 7.8]. 2) Probably, there is some problem with pH adjustment for pH 5.5. P. pyralis luciferase should give nearly monomodal red spectrum at pH 5.5, maybe with a faint green shoulder (Branchini BR, 2007, Oba Y, 2012, Riahi-Madvar A, 2009). Here it is only a faint red shoulder.