On 2017 Jul 31, George McNamara commented:

Western (blot) misconduct paper: not perfect, but worth reading and thinking about. I'll also add that: * in this era of NGS (next generation sequencing), any RNAseq or single cell RNAseq that does not pass all the filters/gates, is going to be ignored ... enabling the authors to "prove their hypothesis (again and again)". * More NGS = fewer Northern blots. Of course Northern blots are just as susceptible to cropping = misconduct as Western blots. * the article mentions insulin, but came out sooner than this paper: https://www.ncbi.nlm.nih.gov/pubmed/28263308 Kracht et al 2017 Autoimmunity against a defective ribosomal insulin gene product in type 1 diabetes. Nature Medicine ... insulin alternative reading frame mRNA results in "a highly immunogenic polypeptide" targeted by cytolytic CD8 T-cells.

On 2017 Jul 31, George McNamara commented:

Western (blot) misconduct paper: not perfect, but worth reading and thinking about. I'll also add that: * in this era of NGS (next generation sequencing), any RNAseq or single cell RNAseq that does not pass all the filters/gates, is going to be ignored ... enabling the authors to "prove their hypothesis (again and again)". * More NGS = fewer Northern blots. Of course Northern blots are just as susceptible to cropping = misconduct as Western blots. * the article mentions insulin, but came out sooner than this paper: https://www.ncbi.nlm.nih.gov/pubmed/28263308 Kracht et al 2017 Autoimmunity against a defective ribosomal insulin gene product in type 1 diabetes. Nature Medicine ... insulin alternative reading frame mRNA results in "a highly immunogenic polypeptide" targeted by cytolytic CD8 T-cells.