On 2017 Aug 04, Henri de la Salle commented:

This review privileges the view that mRNAs are translated in platelets. However, the biological function of mRNAs in platelets is not so clear. Our works do not agree with the conclusions drawn from other ones quoted in this review. We have demonstrated (Angénieux et al., PLoS One. 2016 Jan 25;11(1):e0148064. doi: 10.1371/journal.pone.0148064) that the lifespan of mRNAs and rRNAs in platelets is reduced, only a few hours. Accordingly, the translation activity in platelets rapidly decay, within a few hours. Thus, in vivo, translation of non-mitochondrial mRNAs only occurs in young platelets, a few percent of blood platelets in physiologic conditions. Most of the works reporting translation in platelets should be revisited by quantifying the number of transcripts of interest actually present in platelets. The RNAscope technique is powerful to investigate this problem; our works indicated that the most frequent transcript (eg beta actin mRNA) can be detected most if not all young platelets, but in only few percent of total blood platelets in homeostatic conditions. Finally, the biological role of translation in young platelets need to be established using accurate quantification methods, which is not easy with these cells.

On 2017 Aug 04, Henri de la Salle commented:

This review privileges the view that mRNAs are translated in platelets. However, the biological function of mRNAs in platelets is not so clear. Our works do not agree with the conclusions drawn from other ones quoted in this review. We have demonstrated (Angénieux et al., PLoS One. 2016 Jan 25;11(1):e0148064. doi: 10.1371/journal.pone.0148064) that the lifespan of mRNAs and rRNAs in platelets is reduced, only a few hours. Accordingly, the translation activity in platelets rapidly decay, within a few hours. Thus, in vivo, translation of non-mitochondrial mRNAs only occurs in young platelets, a few percent of blood platelets in physiologic conditions. Most of the works reporting translation in platelets should be revisited by quantifying the number of transcripts of interest actually present in platelets. The RNAscope technique is powerful to investigate this problem; our works indicated that the most frequent transcript (eg beta actin mRNA) can be detected most if not all young platelets, but in only few percent of total blood platelets in homeostatic conditions. Finally, the biological role of translation in young platelets need to be established using accurate quantification methods, which is not easy with these cells.