On 2017 Oct 26, Duke RNA Biology Journal Club commented:

This paper was chosen based on the well written abstract. The observation in the title appeared interesting and novel with implications for a role of miRNA in epigenetics with response to environmental conditions. However, upon further reading and analysis of the paper, we expect more work will be done to solidify these initial conclusions. We agree that miRNAs are divergent when flies are grown at different temperatures, additionally, piRNAs do seem to be expressed more at lower temperatures. That this holds true when flies are subsequently switched to different temperatures was also fascinating. However, this paper remained mostly observational. We expected, from general patterns observed in narratives of other papers, that the authors would attempt looking at the protein expression of machinery associated with each process, miRNA and piRNA processing, at each temperature to help elucidate a mechanism for these observations. However, what followed was a speculative section on these observations based on RNA-expression, which does not always correlate with protein expression. Overall, we think this article provides a great starting point for future work involving the functional impact that these molecular changes to environmental stimulus can have on an organism.

On 2017 Oct 26, Duke RNA Biology Journal Club commented:

This paper was chosen based on the well written abstract. The observation in the title appeared interesting and novel with implications for a role of miRNA in epigenetics with response to environmental conditions. However, upon further reading and analysis of the paper, we expect more work will be done to solidify these initial conclusions. We agree that miRNAs are divergent when flies are grown at different temperatures, additionally, piRNAs do seem to be expressed more at lower temperatures. That this holds true when flies are subsequently switched to different temperatures was also fascinating. However, this paper remained mostly observational. We expected, from general patterns observed in narratives of other papers, that the authors would attempt looking at the protein expression of machinery associated with each process, miRNA and piRNA processing, at each temperature to help elucidate a mechanism for these observations. However, what followed was a speculative section on these observations based on RNA-expression, which does not always correlate with protein expression. Overall, we think this article provides a great starting point for future work involving the functional impact that these molecular changes to environmental stimulus can have on an organism.