On 2017 Jun 29, Edward Morrow commented:

The authors state "Hyslop et al. (2016) demonstrated that global gene expression in early PNT blastocysts did not differ from unmanipulated controls".

Morrow & Ingleby (2017) demonstrate why the size and experimental design of Hyslop et al (2016) cannot provide conclusive evidence that expression profiles of manipulated or control samples are indistinguishable from one another, due to low power.

References

Hyslop et al. (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534:383–386. doi:10.1038/nature18303 Hyslop LA, 2016

Morrow EH and Ingleby FC (2017) Detecting differential gene expression in blastocysts following pronuclear transfer. BMC Res Notes. 2017 Feb 15;10(1):97 Morrow EH, 2017 DOI: 10.1186/s13104-017-2421-3

On 2017 Jun 29, Edward Morrow commented:

The authors state "Hyslop et al. (2016) demonstrated that global gene expression in early PNT blastocysts did not differ from unmanipulated controls".

Morrow & Ingleby (2017) demonstrate why the size and experimental design of Hyslop et al (2016) cannot provide conclusive evidence that expression profiles of manipulated or control samples are indistinguishable from one another, due to low power.

References

Hyslop et al. (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534:383–386. doi:10.1038/nature18303 Hyslop LA, 2016

Morrow EH and Ingleby FC (2017) Detecting differential gene expression in blastocysts following pronuclear transfer. BMC Res Notes. 2017 Feb 15;10(1):97 Morrow EH, 2017 DOI: 10.1186/s13104-017-2421-3