On 2017 Jul 04, Parmit Singh commented:

Very interesting finding that sad-7 is showing localization in cytoplasm, peripheral and in nuclear regions. Understanding the correlation of localization with its function in meiotic silencing might be interesting. Interestingly, its expression is similar to sad-4. Also like sad-4, it produces 50% round spores in a heterozygous cross. Homozygous cross is barren in case of sad-7 whereas it is very less productive in case of sad-4. In case of sad-5 and sad-6 (both sad-5 and sad-6 are nucleus localized suppressors, homozygous crosses are fertile. This suggests that the meiotic function of sad-7 might be related to its perinuclear localization or peripheral part of meiotic silencing similar to sad-4. This is further supported by the fact that recently identified two other suppressors cbp20 and cbp80 (cap - binding proteins) produce 80% round spores (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386863/pdf/1149.pdf). Proteins of both genes cbp20 and cbp80 are localized in nucleus and homozygous crosses are fertile like sad-4.

On 2017 Jul 04, Parmit Singh commented:

Very interesting finding that sad-7 is showing localization in cytoplasm, peripheral and in nuclear regions. Understanding the correlation of localization with its function in meiotic silencing might be interesting. Interestingly, its expression is similar to sad-4. Also like sad-4, it produces 50% round spores in a heterozygous cross. Homozygous cross is barren in case of sad-7 whereas it is very less productive in case of sad-4. In case of sad-5 and sad-6 (both sad-5 and sad-6 are nucleus localized suppressors, homozygous crosses are fertile. This suggests that the meiotic function of sad-7 might be related to its perinuclear localization or peripheral part of meiotic silencing similar to sad-4. This is further supported by the fact that recently identified two other suppressors cbp20 and cbp80 (cap - binding proteins) produce 80% round spores (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386863/pdf/1149.pdf). Proteins of both genes cbp20 and cbp80 are localized in nucleus and homozygous crosses are fertile like sad-4.