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  1. Apr 2019
  2. www.neb.com www.neb.com
    PCR Using Q5® High-Fidelity DNA Polymerase (M0491) | NEB
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    1. pbk1 23 Apr 2019
      10–30 second annealing step at 3°C above the Tm of the lower Tm primer.
      Q5-polymerase-Ta
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    neb.com/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491