2 Matching Annotations
- Nov 2021
-
-
• Relative quantitation — The target is compared to an internal standard,using either the standard curve or comparative CT method.• Absolute quantitation — The CT of the unknown samples is comparedagainst a standard curve with known copy numbers.MethodsAnalyze results
Relative and Absolute quantification
-
Use 1–10 ng single-stranded cDNA or 10–100 ng gDNA per reactio
cDNA requirements for Power-up SYBR qPCR
-