bind the transposon DNA at the flanking inverted repeat sequences, excise the transposon, and paste it into a random TA dinucleotide on a target DNA

piggyBac transposon has a broad host spectrum from yeast to mammals, this mobile element has been widely used for a variety of applications in a diverse range of organisms

homologs of the Tn7 transposition proteins, in highly diverged bacteria adapted to a remarkable number of different environments