Call up her father
When Iago says "Call up her father," it is the beginning of his manipulation and how he gets others to be tools of his own cunning devices. On the surface, the line can be interpreted as simply an order, but in fact, it shows how cunning and devious Iago is. Instead of confronting Othello or Desdemona himself, he uses Roderigo to set things on fire for him. By waking Brabantio at midnight, Iago knows that he will be provoking anger, confusion, and fright, emotions that make humans vulnerable to manipulation. This is significant because it makes clear that Iago's evil does not result from aggression but from his ability to provoke reaction and manipulate feelings. He is aware of how to use timing and fright to make fighting break out in moments of peace, and this line alone is the start of that. It also shows one of the prevailing motifs of Othello: the idea that destruction is oftentimes started quietly in suggestion and manipulation and not in outright brutality. By having others "call up" the trouble for him, Iago gets to preserve his good name while the damage is being done, highlighting how risky lying can be when clothed in peaceful words.