5 Matching Annotations
  1. Dec 2020
    1. Between them, the three buffers cover all of the molecular biologist’s DNA gel needs
  2. gmo-crl.jrc.ec.europa.eu gmo-crl.jrc.ec.europa.eu
    1. Since most of the power produced in the electrophoretic process is dissipated as heat the following detrimental effects can result: • an increased rate of diffusion of sample and buffer ions leading to broadening of the separated samples • the formation of convection currents, which leads to mixing of separated samples; • thermal instability of samples that are rather sensitive to heat (e.g. denaturation of DNA) • a decrease of buffer viscosity hence a reduction in the resistance of the medium
    1. Using a lower concentration running buffer (0.25x TAE) and higher voltage (300 V), agarose gels can be run 33% faster.
  3. Jan 2020
    1. Borate, which is present in LB and SB, causes reversible crosslinks with DNA, reducing the separation of larger DNA fragments unless low % gels are made of high-strength agarose
  4. Dec 2019
    1. SDS in loading buffer may contribute to band smearing in precast GelRed® gels. If this occurs, we recommend using the post-staining protocol