2 Matching Annotations
- Jan 2020
- Dec 2019
A single transformant may have a mutation at a low level that will eventually sweep through the population
How does transforming a pure plasmid (miniprep) produce a mixed population?
- Is a single colony on the transformation plate not really clonal due to evolution occurring in growth from the single founder cell?
- And also the outgrowth steo prior to plating is introducing additional variation that differentiates different colonies
- Hence adding these two statements together, picking 3 colonies from a plate streaked from a glycerol stock (derived from a clonal population) has lower variability than 3 colonies picked from a fresh transformation?
Does this make any suggestion of using one or the other method for generating independent biological replicates?