5 Matching Annotations
  1. Dec 2019
    1. A single transformant may have a mutation at a low level that will eventually sweep through the population

      How does transforming a pure plasmid (miniprep) produce a mixed population?

      • Is a single colony on the transformation plate not really clonal due to evolution occurring in growth from the single founder cell?
      • And also the outgrowth steo prior to plating is introducing additional variation that differentiates different colonies
      • Hence adding these two statements together, picking 3 colonies from a plate streaked from a glycerol stock (derived from a clonal population) has lower variability than 3 colonies picked from a fresh transformation?

      Does this make any suggestion of using one or the other method for generating independent biological replicates?

  2. Sep 2019
  3. Aug 2019
    1. Here, we show how three phenomena—Simpson’s paradox, context-dependence, and nonlinear averaging—can lead to erroneous conclusions about population parameters and species interactions when samples contain heterogeneous mixtures of populations or communities.
    2. These issues can be overcome by measuring and accounting for spatial heterogeneity at very small scales
  4. May 2019