- May 2019
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www.research.manchester.ac.uk www.research.manchester.ac.uk
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Genomic DNA Preparation: Genomic DNA, used as a template for PCR, was isolated from approximately 20 wild-type flies. Flies were added to 125ul Homogenisation buffer (200mM sucrose, 100mM Tris-HCl pH 8.0, 50mM EDTA, 0.5% SDS) and ground using a pestle. The mixture wasthen incubated at 67°C for 10mins. Subsequently, 1.5M KAc was added and incubated on ice for 10mins, followed by DNA extraction using an equal volume of phenol chloroform. The mixture was centrifuged at 16,000g and the DNA precipitated using 0.3M NaAc andethanol. The DNA pellet was then resuspended in 25μl of TE with 25ug RNaseA. PCR:Unless otherwise stated, all PCR reactions were performed using Phusion High Fidelity DNA Polymerase (NEB). PCR reactions were carried out at either 20μl or 50μl with the following reaction setup: 1x GC or HF Buffer, 200μM dNTPs, 0.5 μM of both primers, 1 Unit of Phusion and a maximum of 200ng of DNA. Thermocycling conditions used were as per the manufacturers instructions with a minimum of 35 PCR cycles at an elongation rate of 30s/kb at 72°C. Elongation time was adjusted as appropriate for the PCR product. Where necessary Tm was optimised using gradient PCR. All PCR reactions were performed on a BIO-RAD T100 Thermal Cycler. Both PCR purification and Gel extraction were performed using the NucleoSpin Gel & PCR Clean up kit (Macherey-Nagel), as per the manufacturers instructions. Unless otherwise specified, all primers used in this thesis were designed using NCBI’s Primer-BLAST, selecting against any primers or primer pairs that would produce unspecific products (Ye et al., 2012).
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Molecular Biology Protocols
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Experimental Methods
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- Aug 2018
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intarch.ac.uk intarch.ac.uk
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Open to Exploration
Internet Archaeology is trialling a new feature by Hypothes.is which enables everyone to make annotations on journal content. To get started, just select any text in any article and add your annotation for everyone to see (Annotations are public by default but highlights are private, visible only to you when you’re logged in to your Hypothes.is account). You can even share your annotations on social media,
Try this for more info https://web.hypothes.is/blog/varieties-of-hypothesis-annotations-and-their-uses/
I'm interested to see how everyone uses it!
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URL
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intarch.ac.uk intarch.ac.uk
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Internet Archaeology is trialling a new feature by Hypothes.is which enables everyone to make annotations on journal content. To get started, just select any text in any article and add your annotation for everyone to see (Annotations are public by default but highlights are private, visible only to you when you’re logged in to your Hypothes.is account). You can even share your annotations on social media,
Try this for more info https://web.hypothes.is/blog/varieties-of-hypothesis-annotations-and-their-uses/
I'm interested to see how everyone uses it!
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- Dec 2017
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www.scienceintheclassroom.org www.scienceintheclassroom.org
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To determine whether the Aβ toxicity–limiting effects of p38γ were tau-dependent, we crossed APP23.p38γ−/− with tau−/− mice.
The basic question for Fig. 2: Is the protective effect that p38y has against Amyloid beta toxicity only seen in mice that have tau proteins. Experiments were ran on mice that had various combinations of p38y and tau.
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To test whether p38γ−/− augments Aβ-induced deficits, we crossed p38γ−/− with Aβ-forming APP23 mice.
Fig 1 C-J experiments are testing to see if the abscence of p38y increases amyloid beta deficiets. Mice that have amyloid beta deficets were bred with mice that did not have p38y kinase. Amyloid beta - forming APP23 mice have amyloid beta induced deficits.
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We used mice with individual deletion of p38α, p38β, p38γ, or p38δ
Each mouse had one isoform of the p38 kinase eliminated from its cellular biology. All other forms are present.
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To understand the roles of p38 kinases in AD, we induced excitotoxic seizures with pentylenetetrazole (PTZ), an approach widely used for studying excitotoxicity in AD mouse models
The basic goal of the research was to understand the role of p38 kinases in AD mice. The experiment used mice that were made to have excitotoxic seizures through injection of pentylenetetrazole (PTZ). Injection of PTZ has been used in the past to study excitotoxicity in AD mice models.
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- Jan 2017
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static1.squarespace.com static1.squarespace.com
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prospective interviewee,
Just a side spiel: In terms of an interviewee and data, everything really is data. I'll be interviewing freshmen next semester with other SLU students and some things I have already told the group to take note of in notebooks (ha ha) are the different responses the interviewee gives. In a way, the sad little freshmen turn into our experiment. Everyone in the group records a different response. These responses include the obvious oral responses, body language, and tone of voice.
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- Dec 2016
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Written by rchase, feel free to edit. It's an experiment.
Since I took the notes using hypothesis, the password to edit is hypothesis.
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- Sep 2016
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scienceintheclassroom.org scienceintheclassroom.org
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EDA (ectodysplasin A)
This is a protein involved in cell signalling between two layers of skin (ectoderm and mesoderm) , which is especially important in embryo formation.
This allows the formation of hair follicles, sweat glands, and teeth.
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- May 2016
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smgprojects.github.io smgprojects.github.io
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all editions
6600 files were downloaded; 333 files appear to be these missing editions with the placeholder text. I have not yet manually verified all of this... which is partly the point, right?
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each text file
6267 print editions, from 1893 - 2010
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.75 range
that is, from .72 to .9. They all have the same placeholder text, but the quality of the ocr makes some consistent errors, which is interesting.
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- Jan 2016
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www.tolerance.org www.tolerance.org
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After some classroom experimentation, a more specific list of smart technology use criteria emerged
As we transform the "HOW" of what we do to support schools, encouraging safe fail experimentation will be important. The design process supports this and iteration consists of success and failure.
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This template essentially encouraged the participating teachers to do—and document—action research.
I think this "formalizing" of teacher and student experimentation is really important. Ensuring that this newfound knowledge (for better or worse) is shared with colleagues is pivotal.
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