- Last 7 days
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developer.mozilla.org developer.mozilla.org
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CSS Grid Layout excels at dividing a page into major regions or defining the relationship in terms of size, position, and layer, between parts of a control built from HTML primitives.
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css-tricks.com css-tricks.com
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Making literal grids. Like X columns with Y gap between them homegrown framework stuff. grid-gap is wonderful, as gutters are the main pain point of grid systems.
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- Jan 2021
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www.donielsmith.com www.donielsmith.com
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Depending on what other component libraries you’ve used, you may be used to handling events by passing callback functions to component properties, or using a special event syntax – Svelte supports both, though one is usually more appropriate than the other depending on your situation. This post explains both ways.
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outline.com outline.com
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Les meilleures applications en ligne de 2020
theme utile; notons les applications : Notion, Bublup, Raindrop, Xodo, Enregistré dans evernote
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- Dec 2020
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www.nature.com www.nature.com
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Allen. W. E. (2020) Population-scale longitudinal mapping of COVID-19 symptoms, behaviour and testing. Nature human behaviour. Retrieved from: https://www.nature.com/articles/s41562-020-00944-2?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+nathumbehav%2Frss%2Fcurrent+%28Nature+Human+Behaviour%29
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www.techieapps.com www.techieapps.com
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A perfect web application idea must be a combination of functionalities and amazing designing elements. To make it more clear, let us first discuss what makes a difference between a great web application and an average one.
it is essential that your web app idea is appropriate in order to achieve your targeted goals. Not only focus on the designing part of your web application, but seamless backend development is important.
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www.scientificamerican.com www.scientificamerican.com
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US, J. R. E., Christian Meissner,Deborah Goldfarb,Ian Jason Lee,The Conversation. (n.d.). New DIY Contact Tracing App Is Based on the Science of Memory. Scientific American. Retrieved December 10, 2020, from https://www.scientificamerican.com/article/new-diy-contact-tracing-app-is-based-on-the-science-of-memory/
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Julienne, H., Lavin, C., Belton, C., Barjakova, M., Timmons, S., & Lunn, P. D. (2020, December 3). Behavioural pre-testing of COVID Tracker, Ireland’s contact-tracing app. https://doi.org/10.31234/osf.io/9cguq
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psyarxiv.com psyarxiv.com
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Albrecht, R., Jarecki, J. B., Meier, D., & Rieskamp, J. (2020, December 1). Risk Preferences and Risk Perception Affect the Acceptance of Digital Contact Tracing. https://doi.org/10.31234/osf.io/4x5f3
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- Nov 2020
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www.reddit.com www.reddit.com
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I wouldn't use Flutter for web, mobile is good though.
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It's super promising for web apps, just maybe not for web pages. I went from React to Svelte to Flutter for my current app project, and every step felt like a major upgrade.Flutter provides the best developer experience bar none, and I think it also has the potential to provide the best user experience. But probably only for PWAs, which users are likely to install anyway. Or other self-contained experiences, like Facebook games. It does have some Flash vibes, but is far more suitable for proper app development than Flash ever was while still feeling more like a normal website to the average user. It won't be the right choice for everything, but I believe it will be for a lot of things.
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addevice.io addevice.io
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Want to know how to create a workout app? Learn about trending fitness app types and features, how to make money with such apps, and how much it will cost you.
If you want to know How to Create a Workout App with our team for a free consultation and mobile application estimation.
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Want to know how to outsource app development? Check the article and discover key destinations, business opportunities, and how to avoid costly mistakes.
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- Oct 2020
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www.youtube.com www.youtube.com
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Online Research Tools and Techniques. (2020, September 16). https://www.youtube.com/watch?v=wGWqBtDkOFs
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github.com github.com
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The lodash/fp module promotes a more functional programming (FP) friendly style by exporting an instance of lodash with its methods wrapped to produce immutable auto-curried iteratee-first data-last methods.
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en.wikipedia.org en.wikipedia.org
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One of the significant differences between the two is that a call to a partially applied function returns the result right away, not another function down the currying chain; this distinction can be illustrated clearly for functions whose arity is greater than two.
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Currying and partial function application are often conflated.
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stackoverflow.com stackoverflow.com
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Looks like the problem is that debounce defaults to waiting for 0 ms ... which is completely useless!
It would be (and is) way to easy to omit the 2nd parameter to https://lodash.com/docs/4.17.15#debounce.
Why is that an optional param with a default value?? It should be required!
There must be some application where a delay of 0 is useless. https://www.geeksforgeeks.org/lodash-_-debounce-method/ alludes to / implies there may be a use:
When the wait time is 0 and the leading option is false, then the func call is deferred until to the next tick.
But I don't know what that use case is. For the use case / application of debouncing user input (where each character of input is delayed by at least 10 ms -- probably > 100 ms -- a delay of 0 seems utterly useless.
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www.nature.com www.nature.com
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IJzerman, H., Lewis, N. A., Przybylski, A. K., Weinstein, N., DeBruine, L., Ritchie, S. J., Vazire, S., Forscher, P. S., Morey, R. D., Ivory, J. D., & Anvari, F. (2020). Use caution when applying behavioural science to policy. Nature Human Behaviour, 1–3. https://doi.org/10.1038/s41562-020-00990-w
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2ality.com 2ality.com
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trusktr herman willems • 2 years ago Haha. Maybe React should focus on a template-string syntax and follow standards (and provide options for pre-compiling in Webpack, etc).
Well anywho, there's other projects now like hyperHTML, lit-html, etc, plus some really fast ones: https://www.stefankrause.ne...
React seems a little old now (and the new Hooks API is also resource heavy).
• Share ›  Michael Calkins trusktr • 4 years ago • edited That's a micro optimization. There isn't a big enough difference to matter unless you are building a game or something extraordinarily odd.
• Share › −  trusktr Michael Calkins • 2 years ago True, it matters if you're re-rendering the template at 60fps (f.e. for animations, or for games). If you're just changing views one time (f.e. a URL route change), then 100ms won't hurt at all.
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I don't think we want the cookbook to become a collection of tutorials regarding how to use certain libraries and tools with svelte. A set of more generic recipes or deep dives into topics would probably serve people better and have broader application.
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github.com github.com
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It's designed for Single Page Applications (SPA). If you need Server Side Rendering then consider using Sapper.
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jackwhiting.co.uk jackwhiting.co.uk
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We will also need to make a tweak to our package.json file to ensure that if we reload on a page we have navigated to, for example, /blog, we will not receive a server error and it will load the appropriate route.
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www.npmjs.com www.npmjs.com
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Leverages hash-based routing, which is optimal for SPAs and doesn't require any server-side processing
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www.websoptimization.com www.websoptimization.com
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How much will it cost to build a car wash mobile app?
Looking to develop mobile app for car wash services? Check out this blog to know the cost of developing on demand car wash booking app
Tags
- Car Wash App Development Costs
- Mobile Car Wash App
- Whitelabled Car Wash App Solution
- Cost of developing a Car wash app
- Car Wash Mobile Application
- Car Wash App Development Company
- Car Wash Appointment Software
- Car Wash Mobile App
- On Demand Car Wash Booking App
- Build a car wash mobile app
Annotators
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www.quora.com www.quora.com
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Most of these companies use some framework which includes an IDL (Interface Definition Language), and a transport (typically RPC/HTTP). A few popular ones are Protocol Buffer (used at Google), Thrift (used at Facebook, Twitter, ...), and Avro (used at LinkedIn). These frameworks allow applications to expose its interface in an IDL file (.proto, .thrift, etc.). Applications communicate to other applications by calling services (exposed at the server side using the IDL) over RPC/HTTP. Services can be written in any language supported by the framework. Similarly, clients too can be written in any language. These frameworks also support versioning that allows compatibility of newer implementation of services with older implementations of the clients and vice-versa. Other sophisticated features include compression, efficient serialization/de-serialization, etc. Some smaller companies use just HTTP REST. For details, please see:1: Protocol Buffers2: Apache Thrift3: Apache Avro
Application Integration
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- Sep 2020
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www.javascriptjanuary.com www.javascriptjanuary.com
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As you can see, it uses Javascript label syntax to tell the compiler where recalculation should be carefully managed. And you thought that no one uses labels any more.
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knowledgehubmedia.com knowledgehubmedia.com
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In this article, I’ll be discussing a few important and effective mobile app marketing strategies that can make your launch successful.
In this article, I’ll be discussing a few important and effective mobile app marketing strategies that can make your launch successful.
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In this article, I’ll be discussing a few important and effective mobile app marketing strategies that can make your launch successful.
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www.websoptimization.com www.websoptimization.com
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How Much Does It Cost For Web Application Development?
Discover How Much Does It Cost To Build A Web Application Development. Get The Website Development Cost, Web Developers Rate For Website Development.
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www.csun.edu www.csun.edu
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Online Graduation Application
Online Graduation Application
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- Aug 2020
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academic-oup-com.ezproxy.rice.edu academic-oup-com.ezproxy.rice.edu
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enabling precise control over agronomically-relevant outputs such as flowering time, stress response, or the biosynthesis of added value metabolites.
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www.medrxiv.org www.medrxiv.org
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Ray, E. L., Wattanachit, N., Niemi, J., Kanji, A. H., House, K., Cramer, E. Y., Bracher, J., Zheng, A., Yamana, T. K., Xiong, X., Woody, S., Wang, Y., Wang, L., Walraven, R. L., Tomar, V., Sherratt, K., Sheldon, D., Reiner, R. C., Prakash, B. A., … Consortium, C.-19 F. H. (2020). Ensemble Forecasts of Coronavirus Disease 2019 (COVID-19) in the U.S. MedRxiv, 2020.08.19.20177493. https://doi.org/10.1101/2020.08.19.20177493
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www.nature.com www.nature.com
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Corbett, K. S., Edwards, D. K., Leist, S. R., Abiona, O. M., Boyoglu-Barnum, S., Gillespie, R. A., Himansu, S., Schäfer, A., Ziwawo, C. T., DiPiazza, A. T., Dinnon, K. H., Elbashir, S. M., Shaw, C. A., Woods, A., Fritch, E. J., Martinez, D. R., Bock, K. W., Minai, M., Nagata, B. M., … Graham, B. S. (2020). SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness. Nature, 1–8. https://doi.org/10.1038/s41586-020-2622-0
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osf.io osf.io
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Rice, W. L., & Pan, B. (2020). Understanding drivers of change in park visitation during the COVID-19 pandemic: A spatial application of Big data [Preprint]. SocArXiv. https://doi.org/10.31235/osf.io/97qa4
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www.websoptimization.com www.websoptimization.com
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Why Hire a Mobile App Development Company and Not a Freelancer?
Looking for app developers? Confused between hiring a freelance developer vs app development company? Read this detailed guide and select the best one.
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www.websoptimization.com www.websoptimization.com
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Top 20 React Native Component Libraries to Make Your Journey Easier in 2020
React native component libraries - Discover the 20 Best React Native component libraries, UI, toolkit with latest react native component examples for 2020.
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www.youtube.com www.youtube.com
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MyData vs. COVID-19 calls (2020, June 5) - https://www.youtube.com/playlist?list=PLbpRS19STpXSWs4kTiVEx2KN5CZh6yCYI
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- Jul 2020
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www.websoptimization.com www.websoptimization.com
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AngularJS & Thymeleaf: What, Why & How to Supercharge Your Next Java App Development Project
Thymeleaf vs Angular Development - What to Choose in 2020?. Read the detailed blog about the app development of single page to Multi page app development.
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arxiv.org arxiv.org
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Kaptchuk, G., Goldstein, D. G., Hargittai, E., Hofman, J., & Redmiles, E. M. (2020). How good is good enough for COVID19 apps? The influence of benefits, accuracy, and privacy on willingness to adopt. ArXiv:2005.04343 [Cs]. http://arxiv.org/abs/2005.04343
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www.nber.org www.nber.org
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Chodorow-Reich, G., & Coglianese, J. (2020). Projecting Unemployment Durations: A Factor-Flows Simulation Approach With Application to the COVID-19 Recession (Working Paper No. 27566; Working Paper Series). National Bureau of Economic Research. https://doi.org/10.3386/w27566
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www.websoptimization.com www.websoptimization.com
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Kickstarting Salon App Development for Your Beauty Business (And How to Grow in Challenging Times)
Read Our Latest Blog To Build Your Own Custom Mobile App For Your Hair Salons, Beauty Salons, Massage Centers, Spas, Beauty Parlour.
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Bae, J.-M. (2014). The clinical decision analysis using decision tree. Epidemiology and Health, 36. https://doi.org/10.4178/epih/e2014025
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Seyitoğlu, F., & Ivanov, S. H. (2020). Service robots as a tool for physical distancing in tourism [Preprint]. SocArXiv. https://doi.org/10.31235/osf.io/k3z6m
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hypothes.is hypothes.is
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Top 10 Web Application Frameworks
From a wide variety of Web Application Development Frameworks, it is eternally tough to pick the one framework that is completely satisfied with the project. Here, Top 10 Web Application Frameworks Describe in detail.
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Frimpong, J. A., & Helleringer, S. (2020). Financial Incentives for Downloading COVID–19 Digital Contact Tracing Apps [Preprint]. SocArXiv. https://doi.org/10.31235/osf.io/9vp7x
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Rheault, L., & Musulan, A. (2020). Explaining Support for COVID-19 Cell Phone Contact Tracing. https://doi.org/10.31235/osf.io/8wcgz
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svelte.dev svelte.dev
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Any top-level statement (i.e. not inside a block or a function) can be made reactive by prefixing it with the $: JS label syntax.
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www.newscientist.com www.newscientist.com
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Liverpool, C. W., Jessica Hamzelou, Adam Vaughan, Conrad Quilty-Harper and Layal. (n.d.). Covid-19 news: App identified Leicester as a virus hotspot weeks ago. New Scientist. Retrieved July 3, 2020, from https://www.newscientist.com/article/2237475-covid-19-news-app-identified-leicester-as-a-virus-hotspot-weeks-ago/
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- Jun 2020
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psyarxiv.com psyarxiv.com
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Cheung, M. W.-L. (2020). Meta-Analytic Structural Equation Modeling [Preprint]. PsyArXiv. https://doi.org/10.31234/osf.io/epsqt
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www.reddit.com www.reddit.com
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Hahn, U. (2020, April 27). ”Psychological Science is not yet crisis-ready”. Reddit. https://www.reddit.com/r/BehSciMeta/comments/g8wrgg/psychological_science_is_not_yet_crisisready/fr5g1vl
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AP NEWS. ‘Colombia’s Medellin Emerges as Surprise COVID-19 Pioneer’, 13 June 2020. https://apnews.com/b3f8860343323d0daeef72191b669baf.
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www.sciencedirect.com www.sciencedirect.com
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Camacho, D., Panizo-LLedot, Á., Bello-Orgaz, G., Gonzalez-Pardo, A., & Cambria, E. (2020). The Four Dimensions of Social Network Analysis: An Overview of Research Methods, Applications, and Software Tools. Information Fusion. https://doi.org/10.1016/j.inffus.2020.05.009
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featuredcontent.psychonomic.org featuredcontent.psychonomic.org
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Forscher, P., Vazire, S., & Anvari, F. (2020, May 19). To create social good, psychology needs credible evidence. Psychonomic Society Featured Content. https://featuredcontent.psychonomic.org/to-create-social-good-psychology-needs-credible-evidence/
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- May 2020
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thehardestscience.com thehardestscience.com
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sciencebusiness.net sciencebusiness.net
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What’s the COVID-19 re-entry plan? Experts debate Europe’s tricky road ahead. (n.d.). Science|Business. Retrieved April 20, 2020, from https://sciencebusiness.net/news/whats-covid-19-re-entry-plan-experts-debate-europes-tricky-road-ahead
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www.stiftung-nv.de www.stiftung-nv.de
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Dark Patterns: Regulating Digital Design. (2020, May 13). https://www.stiftung-nv.de/en/publication/dark-patterns-regulating-digital-design
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theconversation.com theconversation.com
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Freyens, B., & Hawkins, J. (n.d.). Contact tracing apps: A behavioural economist’s guide to improving uptake. The Conversation. Retrieved May 4, 2020, from http://theconversation.com/contact-tracing-apps-a-behavioural-economists-guide-to-improving-uptake-137157
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www.theguardian.com www.theguardian.com
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editor, P. B. S. policy. (2020, May 3). NHS coronavirus crisis volunteers frustrated at lack of tasks. The Guardian. https://www.theguardian.com/world/2020/may/03/nhs-coronavirus-crisis-volunteers-frustrated-at-lack-of-tasks
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twitter.com twitter.comTwitter1
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ReconfigBehSci en Twitter: “a google doc tracking all app development: https://t.co/vzmyWWF0qj” / Twitter. (n.d.). Twitter. Retrieved April 16, 2020, from https://twitter.com/SciBeh/status/1243171088256860160
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gitlab.com gitlab.com
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Could one solution design serve the needs of multiple persona scenarios?
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github.com github.com
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Not building a static site? No worries there, React Static works as an SPA too, even if there is only a single index.html file.
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www.excellentwebworld.com www.excellentwebworld.com
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Online Video Streaming Application
Netflix app is one of the most downloaded & popular videos streaming apps across the globe. Looking at its success we have kept it our App of the Week space.
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en.wikipedia.org en.wikipedia.org
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jamstack.org jamstack.org
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the expanding selection of CMS options for site generators remove the need to maintain a separate stack for content and marketing.
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www.termsfeed.com www.termsfeed.com
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The GDPR is a little short on examples, but this is always interpreted very broadly.
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en.wikipedia.org en.wikipedia.org
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In mathematical contexts, duality has numerous meanings[1] although it is "a very pervasive and important concept in (modern) mathematics"[2] and "an important general theme that has manifestations in almost every area of mathematics".[3]
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psyarxiv.com psyarxiv.com
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Bailon, C., Goicoechea, C., Baños, O., Damas, M., Pomares, H., Correa, Á., Sanabria, D., & Perakakis, P. (2020). CoVidAffect: Real-time Monitoring of Mood Variations Following the COVID-19 Outbreak [Preprint]. PsyArXiv. https://doi.org/10.31234/osf.io/3sv6r
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www.iubenda.com www.iubenda.com
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This scope effectively covers almost all companies and, therefore, means that the GDPR can apply to you whether your organization is based in the EU or not.
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www.iubenda.com www.iubenda.com
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You can choose from “Apply GDPR’s broader protection standards to” EU onlyAll users
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www.samajinfotech.com www.samajinfotech.com
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Samaj Infotech is dedicated to delivering dynamic solutions as an honoured company for Web Application Development Services USA.
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- Apr 2020
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medium.com medium.com
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Isomorphism itself is not purely a JavaScript thing. You can also build an isomorphic application in Dart or even use two different languages/stacks to render the same result. In fact there are many “isomorphic” applications which render a full HTML document on the server using PHP, Java or Ruby and use JavaScript and AJAX to create a rich user experience in the browser. This was the way things were done before the rise of Single Page Applications. The only real reason isomorphism is tied to JavaScript is because it’s the only language widely supported in the browser, and so at least part of the whole thing will use JavaScript. There’s no such thing as Isomorphic JavaScript, only isomorphic applications.
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www.excellentwebworld.com www.excellentwebworld.com
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COVID-19 Tracking Mobile Application
Arogya Setu is an app created by the Government of India for the people of India to fight against and prevent themselves from getting infected with COVID-19.
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IJzerman, H., Lewis, N. A., Jr., Weinstein, N., DeBruine, L. M., Ritchie, S. J., Vazire, S., … Przybylski, A. K. (2020, April 27). Psychological Science is Not Yet a Crisis-Ready Discipline. https://doi.org/10.31234/osf.io/whds4
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vocalishealth.com vocalishealth.com
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Vocalis Health. (n.d.). Retrieved April 23, 2020, from https://vocalishealth.com/
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www.excellentwebworld.com www.excellentwebworld.com
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Web Application Architecture – Its Components & TypesYou are here:HomeApps & SoftwareWeb Application Architecture – Its…
A web application that works on the remote server with the help of the internet. The app is stored on the remote server and gets delivered over the browser interface.
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drive.google.com drive.google.com
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Joint Statement.pdf. (n.d.). Google Docs. Retrieved April 21, 2020, from https://drive.google.com/file/d/1OQg2dxPu-x-RZzETlpV3lFa259Nrpk1J/view?usp=drive_open&usp=embed_facebook
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www.abc.net.au www.abc.net.au
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Deputy CMO doesn’t rule out forcing Australians to download contact tracing app. (2020, April 17). [Text]. ABC News. https://www.abc.net.au/news/2020-04-17/paul-kelly-coronavirus-tracing-app/12158854
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github.com github.com
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Devise-Two-Factor only worries about the backend, leaving the details of the integration up to you. This means that you're responsible for building the UI that drives the gem. While there is an example Rails application included in the gem, it is important to remember that this gem is intentionally very open-ended, and you should build a user experience which fits your individual application.
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www.w3.org www.w3.org
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It will allow anyone to annotate anything anywhere, be it a web page, an ebook, a video, an image, an audio stream, or data in raw or visualized form
Tags
Annotators
URL
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theconversation.com theconversation.com
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L’application Affluences, Grand Prix de l’Innovation Digitale 2018 dans la catégorie « Ville Connectée », permet de suivre en temps réel le taux d’occupation des musées partenaires et le temps d’attente pour y accéder.
Argument en faveur de l'utilisation du numérique sous forme d'applications. Argument épistémique inductif (Cette appli numérique permet de réduire l'attente donc le numérique est une solution au problème)
// nous émettons des réserves quant à l'enthousiasme que suscite la notion de "ville connectée" https://www.lepoint.fr/editos-du-point/laurence-neuer/ville-intelligente-le-risque-est-que-les-donnees-se-retournent-contre-les-personnes-03-11-2017-2169630_56.php
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- Mar 2020
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hackernoon.com hackernoon.com
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Single Page Applications: The Rise of Web Apps in 2020
If you want an excellent user engagement between your application and them, single page web apps is the best choice. To offer the best real-time experience to the users, web apps like Google-Maps use single page application.
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yourstory.com yourstory.com
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How Single Page Application Can Benefit Your Business?
The definition of single-page web apps lies exactly in their words. It has only one page, which means the whole page gets load only for one time. Later when users request any data only that data will be updated not the whole page.
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- Feb 2020
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www.excellentwebworld.com www.excellentwebworld.com
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Single page web apps are an ideal choice when thinking about future web development. This architecture is a perfect choice for social networks stuff, SaaS platforms, or some close communities where SEO doesn’t matter.
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- Dec 2019
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www.nairaland.com www.nairaland.com
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with how Much Can I create An Online Retail Website And APP?
If you want to know the actual cost of your application then you can refer to article. From this guide, you can get all the features cost and overall cost. You can also get a few tips about how you can decrease the cost up to 45%. You can also get the answer to a few famous mobile application clone solutions.
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vnnovate.com vnnovate.com
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iPad App Development Company
At being a bespoke iPad App Development company - Vnnovate Solutions, we’re strategists and full stack iPad app developers, who know how to develop latest iOS 12 compatible mobile applications and deliver comprehensive iOS app development services.
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www.spreaker.com www.spreaker.com
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How much does it cost to make an app in 2019-20
Uber, Ola, Tiktok, Instagram and infinity number of apps became an inspiration for businesses to start a business online. You can also get ideas about what it cost to make a clone application like Instagram, Tiktok, Spotify, Netflix and much more.
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academic.oup.com academic.oup.com
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sequential lineage tracing studies aimed at unraveling the relationships between cellular precursors and mature cell types
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- Nov 2019
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www.biorxiv.org www.biorxiv.org
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It is challenging to study how pneumococci control virulence factor expression, because cues of natural environments and the presence of an immune system are difficult to simulate in vitro
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- Oct 2019
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www.biorxiv.org www.biorxiv.org
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We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes
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www.sciencedirect.com www.sciencedirect.com
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This paper explores how the Internet of Things and blockchain technology can benefit shared economy applications. The focus of this research is understanding how blockchain can be exploited to create decentralised, shared economy applications that allow people to monetise, securely, their things to create more wealth. Shared economy applications such as Airbnb and Uber are well-known applications, but there are many other opportunities to share in the digital economy. With the recent interest in the Internet of Things and blockchain, the opportunity exists to create a myriad of sharing applications, e.g. peer-to-peer automatic payment mechanisms, foreign exchange platforms, digital rights management and cultural heritage to name but a few
This article sheds light into shared economy, have highlighted just the abstract, but this entire article is so interesting and the application it talks about is great, like how can airbnb or uber use blockchain to protect it's clients privacy. Must read
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vnnovate.com vnnovate.com
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iPhone App Development Company
Best iPhone App Development company Sydney, delivering bespoke and adaptable iPhone App Development services to SMEs, enterprises & startups across the Australia.
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www.annualreviews.org www.annualreviews.org
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A technique common in rodents is the use of Cre recombinase lines that are inducible at specific developmental time points (Figure 3b). The most common form of inducible Cre is CreERT2, which contains a modified estrogen receptor binding domain that prevents Cre from entering the nucleus in the absence of a ligand
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www.nature.com www.nature.com
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creating improved technologies for large-scale recordings of neural activity in the live brain is a crucial goal in neuroscience
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www.frontiersin.org www.frontiersin.org
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new toolkits for chronic labeling of active ensembles will provide a much awaited experimental basis to interrogate various aspects of neuronal circuits underlying long-term plastic changes of the brain, such as during nervous system development, during establishment of long-lasting remote memory over months, or in association with age-related neuronal changes over several years.
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new direction of functional labeling involves conversion of transient expression from activity-dependent promoters into a permanent labeling based on tamoxifen-dependent recombinases
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This gene fusion approach will allow us to assay the induction of gene expression in as few as one cell
Recombinase memory as a reporter for expression in 1 cell
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pubs-acs-org.ezproxy.rice.edu pubs-acs-org.ezproxy.rice.edu
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Because growth conditions (i.e., fluid dynamics and nutrient composition) can also have a profound effect on when QS is important,(71-75) there is a need to study biofilm formation and QS of BNR bacteria under various potential operating conditions.
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www.nature.com www.nature.com
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difficult to observe in situ at the microscale, hence mechanisms and time scales relevant for bacterial spatial organization remain largely qualitative.
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aem.asm.org aem.asm.org
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For in situ studies of AHL-dependent signaling in complex habitats, biosensors utilizing fluorescent proteins (GFP and RFP) as reporter genes have been developed
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biosensors were employed to demonstrate AHL-mediated communication in swarm colonies of Serratia liquefaciens
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www.pnas.org www.pnas.org
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it is unclear to what extent these results are relevant in natural habitats, as the standard assays neglect the different surface chemistries, interactions with other species, and physical constraints of natural environments.
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- Sep 2019
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www.pnas.org www.pnas.org
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This biosensor will help identify organic substrates that potentially support microbial growth and activity before and during nodulation
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Such biosensors can reveal intriguing aspects of the environment and the physiology of the free-living soil S. meliloti before and during the establishment of nodulation, and they provide a nondestructive, spatially explicit method for examining rhizosphere soil chemical composition
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besjournals.onlinelibrary.wiley.com besjournals.onlinelibrary.wiley.com
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quantifying biogeochemical fluxes resulting from these reactions remains a challenge
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These tools provide insights into processes such as N uptake at the scale of individual root tips, allowing observation of plant–microbe interactions on scales at which they actually occur, instead of being masked by a whole‐core average
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www.sciencedirect.com www.sciencedirect.com
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more convenient and effective methods is required for qualitative and quantitative analysis of AHLs
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blogs.ei.columbia.edu blogs.ei.columbia.edu
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Now synthesized microbial biosensors are able to target specific toxins such as arsenic, cadmium, mercury, nitrogen, ammonium, nitrate, phosphorus and heavy metals, and respond in a variety of ways. They can be engineered to generate an electrochemical, thermal, acoustic or bioluminescent signal when encountering the designated pollutant.
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- Aug 2019
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news.rice.edu news.rice.edu
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Now we can ask, ‘Have I seen iron at some threshold?’ ‘Have I seen some nitrate?’ And have every microbe write it down. As one thinks about long-term incubation, this is a really powerful tool, because even six months later we can pull out the DNA and see what happened.”
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phys.org phys.org