See notes in this section for restriction digestion tips

NEB says

Digestion is recommended whenever DNA input is greater than 75 ng Source

NEB says that biorad recommends these enzymes: AluI, CviQI, HaeIII, HindIII-HF, MseI

More guidelines

• Assemble ddPCR reactions at room temperature, this allows the restriction enzyme to digest the gDNA during the reaction set-up period
• Prepare reaction mixes as you would for a standard ddPCR reaction. Add 0.5–1 μL of each restriction enzyme (5–20 units, depending on enzyme concentration) to the reaction mixture
• After set-up, simply continue droplet generation as normal
• Restriction enzyme will be inactivated during first PCR denaturation step

Refer to this protocol mentioned in the BioRad page, annotation

!- For : microbiome health - fasting for 14 hours helps the migrating motor complex (MMC) maintain gut health

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Tom Lawton. (2022, January 10). 🔹99.995% of people who drank from that pump didn’t die. 🔹We need to learn to live with the dirty water. 🔹Dirty water is fine to drink if you don’t have underlying health conditions. 🔹It’s not necessary to clean the water unless it’s wholly waterborne. (/S!) [Tweet]. @LawtonTri. https://twitter.com/LawtonTri/status/1480502910459039749

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This could explain why Dr Clemens' [[PKD]] protocol has me around 1,000 calories a day

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Jeremy Howard. (2021, August 29). I’ve been analyzing the UK covid data and I’ve just discovered something shocking. Cases in chidrens in England have just smashed all-time highs. Nearly double what they’ve ever been before. And rising VERY rapidly. Schools are about to reopen. With far fewer restrictions. Https://t.co/rNhW4U98BR [Tweet]. @jeremyphoward. https://twitter.com/jeremyphoward/status/1432118975060594691

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