181 Matching Annotations
  1. May 2024
    1. DNA fragmentation by restriction digestion prior to dropletgeneration enables optimal accuracy by separating tandemgene copies, reducing sample viscosity, and improving templateaccessibility for input samples >66 ng per well

      NEB says

      Digestion is recommended whenever DNA input is greater than 75 ng Source

      NEB says that biorad recommends these enzymes: AluI, CviQI, HaeIII, HindIII-HF, MseI

      More guidelines

      • Assemble ddPCR reactions at room temperature, this allows the restriction enzyme to digest the gDNA during the reaction set-up period
      • Prepare reaction mixes as you would for a standard ddPCR reaction. Add 0.5–1 μL of each restriction enzyme (5–20 units, depending on enzyme concentration) to the reaction mixture
      • After set-up, simply continue droplet generation as normal
      • Restriction enzyme will be inactivated during first PCR denaturation step
  2. Aug 2022
    1. Mayer says time-restricted eating — a form of intermittent fasting that requires you to squeeze all your daily calories into a compressed feeding window — may be helpful. “The migrating motor complex is rarely mentioned in these articles on intermittent fasting, which is surprising because it’s so well-studied,” he says.To ensure the MMC has enough time to perform its duties, aiming for 14 hours without caloric foods or drinks is a good target, he says. For example, you could avoid all calories between 8 p.m. and 10 a.m. “The 14 hours without food intake would allow the MMC to kick in and not only cleanse your gut of any undigestible, unabsorbable food components, but also to reestablish the normal proximal-to-distal gradient of gut microbial density,” he says.

      !- For : microbiome health - fasting for 14 hours helps the migrating motor complex (MMC) maintain gut health

  3. Apr 2022
  4. Mar 2022
    1. There are a few things we know that promote longevity and healthspan. Caloric restriction and intermittent fasting are one of the most effective ways of doing so as shown by research in virtually all species.

      This could explain why Dr Clemens' [[PKD]] protocol has me around 1,000 calories a day

  5. Jan 2022
  6. Dec 2021
  7. Nov 2021
  8. Oct 2021
  9. Sep 2021
  10. Aug 2021
  11. Jul 2021
  12. Jun 2021
    1. Paul Kedrosky. (2021, June 8). Air passengers in the U.S. yesterday were up 20% week-over-week, the biggest advance since February, and the highest total daily passengers—1.98m—Since the pandemic started. I initially thought it was an error and held off posting it, but it’s apparently correct. Https://t.co/E50EW54MR0 [Tweet]. @pkedrosky. https://twitter.com/pkedrosky/status/1402047355029917696

  13. May 2021
    1. Peccia, J., Zulli, A., Brackney, D. E., Grubaugh, N. D., Kaplan, E. H., Casanovas-Massana, A., Ko, A. I., Malik, A. A., Wang, D., Wang, M., Weinberger, D. M., & Omer, S. B. (2020). SARS-CoV-2 RNA concentrations in primary municipal sewage sludge as a leading indicator of COVID-19 outbreak dynamics. MedRxiv, 2020.05.19.20105999. https://doi.org/10.1101/2020.05.19.20105999

  14. Apr 2021
  15. Mar 2021