32 Matching Annotations
  1. Oct 2020
    1. Repeated freezing and thawing of the supermix is not recommended. DTT should be aliquoted to multiple tubes and stored at –20°C to minimize freezing and thawing
    1. Knight, S. R., Ho, A., Pius, R., Buchan, I., Carson, G., Drake, T. M., Dunning, J., Fairfield, C. J., Gamble, C., Green, C. A., Gupta, R., Halpin, S., Hardwick, H. E., Holden, K. A., Horby, P. W., Jackson, C., Mclean, K. A., Merson, L., Nguyen-Van-Tam, J. S., … Harrison, E. M. (2020). Risk stratification of patients admitted to hospital with covid-19 using the ISARIC WHO Clinical Characterisation Protocol: Development and validation of the 4C Mortality Score. BMJ, 370. https://doi.org/10.1136/bmj.m3339

  2. Sep 2020
    1. What we need instead is a spatial meta layer for notes on the OS-level that lives across all apps and workflows. This would allow you to instantly take notes without having to switch context. Even better yet, the notes would automatically resurface whenever you revisit the digital location you left them at.

      Dit is een interessante gedachte. Ik heb eerste ideeën hoe dit als protocol kan samenhangen met Webmentions, Micropub en Microformats.

  3. Aug 2020
  4. Jul 2020
  5. Jun 2020
    1. MQTT

      Message Queuing Telemetry Transport MQTT protocol -> publish-subscribe network protocol that transports messages between devices, usually through TCP/IP

      Protocol defines 2 network entities:

      1. message broker -> receives messages from clients and then sends them to any clients subscribed to topic.
      2. a number of clients ->
      <table style="border: 1px solid black;"> <tr> <th style="border: 1px solid black;"> MQTT un-encrypted port </th> <th style="border: 1px solid black;"> MQTT encrypted port </th> </tr> <tr> <td style="border: 1px solid black;">**1883** </td> <td style="border: 1px solid black;">**8883** </td> </tr> </table>
    1. e present a protocol for secure online com-munication, called “off-the-record messaging”, which hasproperties better-suited for casual conversation than do sys-tems like PGP or S/MIME.
    1. In 2013, the Signal Protocol was introduced, which is based on OTR Messaging and the Silent Circle Instant Messaging Protocol (SCIMP). It brought about support for asynchronous communication ("offline messages") as its major new feature, as well as better resilience with distorted order of messages and simpler support for conversations with multiple participants.[11] OMEMO, introduced in an Android XMPP client called Conversations in 2015, integrates the Double Ratchet Algorithm used in Signal into the instant messaging protocol XMPP ("Jabber") and also enables encryption of file transfers. In the autumn of 2015 it was submitted to the XMPP Standards Foundation for standardisation.
  6. May 2020
  7. Mar 2020
  8. Jan 2020
  9. Sep 2019
  10. Feb 2019
    1. Research methodologies and methods used must be open for full discussion and review by peers and stakeholders.

      So does this mean totally open? As in publish your protocols open?

  11. Jan 2019
  12. Mar 2018
    1. It would be fair to characterize Beaker as “a novel application of Bittorrent’s concepts to the Web platform.” If Beaker had been started in 2006, it would be using Bittorrent as its primary protocol. However, as of 2016, new variants have appeared with better properties.
  13. Aug 2017
    1. A Technical Perspective in Modern Tree-ring Research - How to Overcome Dendroecological and Wood Anatomical Challenges

      microtome-generated sections along the entire length of wood core samples for anatomical studies

  14. Jun 2017
  15. May 2017
    1. Antoine Amarilli fait remarquer le d ́es ́equilibre apparent de la situation actuelle, o`ul’association devrait payer ORCID pour avoir le droit de leur fournir des donn ́ees. L’AGdemande si d’autres acteurs se trouvent dans une situation analogue `a la nˆotre. Anto-nin Delpeuch rappelle que les outils de gestion de recherche vendus aux universit ́es lesplacent dans une sitation similaire `a la nˆotre ; `a leur ́echelle, cependant, le montant dela cotisation `a ORCID ne repr ́esente pas un probl`eme de la mˆeme ampleur.

      Solid which is a decentralised authentication protocol may be of interest.