Decimal serial dilutions (100–1000 μl) of 101–106 or 107 were prepared from the 100 stock in 1.5 ml tubes with 4–5 repeated flushing and changing of tips
changing tips seems important?
3 Matching Annotations
- Feb 2024
-
www-sciencedirect-com.ezproxy.rice.edu www-sciencedirect-com.ezproxy.rice.edu
-
-
www-nature-com.ezproxy.rice.edu www-nature-com.ezproxy.rice.edu
-
However, none of these approaches combines the simplicity, low cost, dynamic range and versatility of simply diluting cells and then growing drops on solid media as first proposed in ref. 17.
First reference to CFU method from 1938!?
-
-
static-content-springer-com.ezproxy.rice.edu static-content-springer-com.ezproxy.rice.edu
-
Pipet 100 μL of Starting Culture into Dilution 1. Discard tip. Do NOT pipette up and down. Vortex tube for 5-10 secs
serial dilution method. Why should you not pipette up and down? Cells killed due to pipetting stress?
-