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- Apr 2020
Mixed the plate thoroughly and incubated for one hour at room temperature. Then 5 μL development solution was added to each well and the plate was incubated for 1h at room temperature;
the compound and kinase are incubated together for at least 2 hours. And 2 hours is enough for covalent adduct to form. if compound can form covalent adduct with FGFR1, why FGFR1 inhibition IC50 is so poor?