28 Matching Annotations
  1. Jul 2019
  2. Jun 2019
  3. May 2019
    1. 0.67% Yeast Nitrogen Base2% DextroseYeast Carbon Base (YCB):1.17% Yeast CarbonBase1% DextroseCAA:0.67% Yeast Nitrogen Base 2% Dextrose0.6% Casamino acids Plates weremade by adding 2% agar
    2. Yeast Extract-Peptone-Dextrose (YPD):1% Yeast extract2% Peptone 2% DextroseYeast Nitrogen Base (YNB)
    3. Yeast medium
    1. Malachite green reagent
    2. DNA loading dye
    3. Neutralization solution(Solution III)
    4. EMSA Buffer
    5. Blocking buffer: 2% BSA
    6. Stripping Buffer
    7. Blocking Buffer
    8. TBST
    9. Transfer Buffer
    10. Running Buffer
    11. Stacking and resolving AcrylamidegelsResolving gel (10 ml)
    12. 6X protein loading buffer (Laemmlibuffer)
    13. Cell lysis buffer(RIPA Buffer)
    14. For Immunoblotting
    15. Sodium Chloride (NaCl)ComponentsFinal concentrationFor 100 mlNaCl5M29.22gH2Oq.s