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  1. Aug 2019
    1. Sucrosc, 'frizma base, PMSI:, EIYSA, Tween-40TM IPolyoxycthylene (20) sorbitol ~nonopalmitatej. Tween-20TM, Glycerol, a-n~ercaptoethanol (2-ME), 1)imethyl sulphoxide (DMSO), TEMED. Ammoniu~n persulphate (APS), sodium dodecyl sulfate (SIX). Brilliant Blue G, Coomassie Blue, OPD, N-cthylmaleimide (NEM). Bovine Serum Albumin (USA), azetidinc-2-carboxilic acid (Azt). I,-canavanine (L-can). phosphatidylclioline, pl~osphatidylserine, phosphatidylethanolamine. cycloheximidr, 1,-amino acids, 1)-amino acids, valinomycin, CCCP, PercollTM, I,-proline agarose, L-argir~ine agarose, n-octyl-p-D- glucopyranoside, dansylated amino acids and egg-phoshatidylclioli~le (Egg-PC) were purchased from Sigma Chemicals Co. (St. Louis, USA
  2. Jul 2019
    2. Conclusion
    3. ROS ASSAY
    4. In vitro cytotoxic activity of methanolic extract of N. sativafrom different germination phasesduringSRB assay
    5. n vitro cytotoxic activity of methanolic extract of N. sativafrom different germination phasesduringMTT assay
    6. Statisticalanalysis
    7. ROS assay
    8. Cytotoxicity assay by Sulphorhodamine B (SRB) me
    9. Cytotoxicity assay by MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyltetrazolium Bromide)method
    10. Cytotoxicityscreenin
    11. Cell culture
    12. Drugs and chemicals
    13. Preparation of distilled extracts
    14. Harvest of germinated seeds
    15. Germination of N. sativaseeds
    16. Collection of N. sativaseeds
    1. TLC study of alkaloids
    2. TLC study of phenols
    3. TLC study of alkaloids
    4. Activation of TLC plate
    5. Preparation of TLC plate
    6. Study of Phytochemicals by Thin Layer Chromatography (Wagner, and Bladt, 1996)
    7. Test for cardiac glycosides
    8. Test for Terpenoids
    9. Tests for Flavonoids
    10. Tests for Phenolic compounds
    11. Tests for Saponins
    12. Tests for Tannins
    13. Tests for Alkaloids
    14. Test for Sterols
    15. Qualitative study of phytochemicals of N. sativadurin
    16. Inoculum preparation
    17. Clinical bacterial strains used for the study
    18. Preparation of distilled extracts
    19. Harvest of germinated seeds
    20. Germination of N.sativaseeds
    21. Collection of N.sativaseeds
    1. Testing of bioformulations
    2. Preparation of bioformulations and determination of
    3. Validation of bioformulations under laboratory conditions (in vitro
    4. Sequence analysis
    5. DNA sequencing of the 18S rDNA fragment
    6. Purification of PCR product
    7. Analysis of internal transcribed spacer region
    8. RAPDand SSRscoring and data analysis
    9. PCR amplification
    10. Running of gel and visualization of DNA
    11. Determination of the yield
    12. Agarose gel electrophoresis
    13. Qualitative and quantitative estimation of DNA
    14. Determination of the yield
    15. Procedure for DNA isolation
    16. Reagents required for fungal DNA isolationand p
    17. DNA isolation of Trichodermaisolate
    18. Genetic variability analysis through RAPD and SSR
    19. Photography, evaluation and documentation
    20. Procedurefor SDS-PAGE
    21. Materialsrequired for SDS-PAGE
    22. Protein profiling of bioagent through SDS-PAGE
    23. Biochemical analysis (Protein estimation)
    24. Protein estimation through Kjeldahl method
    25. Effect of temperature on xylanase activity
    26. Effect of pH on xylanase activity
    27. Effect of various carbon sourceson xylanase activity
    28. Assayof xylanase activity
    29. Dinitrosalicylate reagent (DNS)(per liter)
    30. Citrate phosphate buffer
    31. Reagents
    32. Xylanase activity
    33. Harvesting of cultures
    34. Enzyme production (EP) medium
    35. Inoculum preparation
    36. Sporulation medium used for Trichodermasp
    37. Maintenance of Trichodermasp. culture
    38. Sterilization
    39. Materials for xylanase induction
    40. Induction of xylanase from Trichodermasp
    41. Evaluation of bioagents against the pathogen
    42. Laboratory screening of antagonists against the test pa
    43. Effect of pHon growth and sporulation of the bioagent
    44. Effect of temperature on growth of bioagent
    45. Effect of different media on growth of bioagentTrichoderma
    46. Cultural characteristicsof bioagent Trichoderma
    47. Mycelial characteristicsof pathogen
    48. Identificationof bioagent
    49. Isolation and purification of Trichoderma sp.
    50. Isolation and purification of pathogen, Fusarium udum
    51. Isolation, purification and morphological characterization of pathogen and bioagent
    52. Symptomatology
    53. Collection of the diseased materia
    54. Sterilization of laminar air flow
    55. Sterilization of media and distilled water
    56. Sterilization of glasswares
    57. Sterilization procedure
    58. Source of chemicals
    59. Experimental site
    1. Relative distribution of virulence genes among V. parahaemolyticusfrom Cochin estuary, shrimp farm and sea food
    2. Prevalence of type III secretion system genes amongV. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
    3. Prevalence of tdhand trhgenes among V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
    4. Relative prevalence of extracellular virulence factors among Vibriofrom Cochin estuary, shrimp farm and seafood
    5. Prevalence of extracellular virulence factors in Vibriofrom seafood
    6. Prevalence of extracellular virulence factors in Vibriofrom shrimp farm
    7. Prevalence of extracellular virulence factors in Vibriofrom Cochin estuary
    8. Gel documentation and image analysis
    9. Screening for virulence genes in V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood
    10. Statistical analysis
    11. Detection of hemolytic activity
    12. Production of caseinase
    13. Production of phosphatase
    14. Bacterial strains used
    15. DNAisolation
    16. Detection of virulence genes tdhand trhby multiplex
    17. Detection of type III secretion system genes
    18. Production of chitinase
    19. Production of DNas
    20. Production of gelatinase
    21. Production of Lipase
    22. Production of amylase
    23. Bacterial strains used
    24. Screening of Vibriostrains for extracellular enzymes
    1. Plasmid curing of Vibriofrom Cochin estuary,shrimp farms and seafood
    2. Plasmid profiles among Vibriofrom Cochin estuary, shrimp farms and seafood
    3. Distribution of antibiotic resistance genes in Vibriofrom Cochin estuary, shrimp farm and seafood
    4. MAR indexingand antibiotic resistance pattern amon
    5. Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood
    6. Antibiotic resistance among Vibriofrom seafood
    7. Antibiotic resistance amongVibriofrom shrimp farm
    8. Antibiotic resistance amongVibriofrom Cochin estu
    9. Antibiotic resistance amongVibrio
    10. Plasmid curing experiment
    11. Plasmid profiling of the drug resistant strains
    12. Gel documentation and image analysis
    13. Detection ofblaNDM-1gene
    14. Detection of blaCTX-Mgene
    15. Detection of blaTEMgene
    16. DNA isolation
    17. Detection of beta-lactam antibiotic resistancegenes
    18. MAR indexing
    19. Antibiotic sensitivity tes
    1. Genbank accession numbers
    2. Seasonal variation in the diversity and distribution of Vibrioin Cochin estuary
    3. Relative diversity and distribution of Vibrioin the water and sediment of Cochin estuary
    4. Species level identification and distribution of Vibriospecies in Cochin estuary
    5. Environmental parameters
    6. Statistical analysis
    7. Gel documentation and image analysis
    8. Detection of toxR gene
    9. Detection of tlhgene
    10. Extraction of genomic DNA
    11. Detection of V. parahaemolyticusspecies-specific gene
    12. Isolation of V. parahaemolyticuson HiCrome Vibrio
    13. Isolation and identification of V. parahaemolyticus
    14. PCR amplification of 16S rRNA gene
    15. DNA isolatio
    16. Molecular confirmation of Vibrioby 16S rRNA gene se
    17. Resistance to ampicillin
    18. Citrate utilisation tes
    19. Nitrate reduction test
    20. Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test
    21. Urease production
    22. Gelatinase production
    23. Growth at different temperatures
    24. Salt tolerance tes
    25. Voges Proskauer (acetoin production) test
    26. Indole production
    27. Carbon source utilisation tes
    28. Carbohydrate fermentation test
    29. Amino acids utilisation test (Decarboxylase/dihydrol
    30. Species level identification
    31. Oxidative-Fermentative test
    32. Oxidase test
    33. Gram staining
    34. Presumptive identification
    35. Isolation of Vibriospecies from water and sediment of Cochin estuary
    36. Sample collection
    37. Analysis of hydrographical parameters