Sonication of bacteria

Irradiation of bacteria

Growth of Bacteria

Tissue Culture Medium

Cell Lines

Mice

Bacteria

PurificationofThermostableDirectHemolysin(TDH)

Bacterialstrain

Cellline

Reagents

Statistical Analysis

Columns for Affinity Chromatography

SOS PAGE

2 Bioassay

Proliferation Assay

Target binding assay

High Performance Liquid Chromatography

Ion Exchange Column Chromatography

IFN Biossay

Cytotoxicity assay

Sephadex G-100 Column Chromatography

Preparation of Mouse MHC-AF

ACK lysis Buffer

ELISA dilution buffer

ELISA blocking buffer

ELISA washing buffer

ELISA binding Buffer

FACS buffer

Youman's modified medium

Phosphate buffered saline (PBS), 0.15M, pH 7.2

Reagents Used

Statistics

Histopathological studies

Bacterial load in the spleens studied by cfu plating

Protection studies

Expression of costimulatory molecules on splenocytes harvested from immunized and control animals

Expression of costimulatory molecules on the vaccines

Phenotypic analysis by Flow cytometry

lgG-isotyping in the sera of the immunized animals

Lymphokines Assay

Lymphoproliferation

Immunization of the animals

Preparation of the vaccines

Cell lines

Bacterial Cultures

Animals

Immunohistochemistry (IHC)

Assay procedure

ELISA

Dot blot assay

Immunodetection

Immunoblotting

Poly acrylamide gel electrophoresis (PAGE

Extraction of total protein from mammalian cells and quantification

RNA extraction and cDNA synthesis

Microscopy

Flow cytometry

Transwell experiment

Animal injection

LV concentration by ultracentrifugation

Preparation of Lentiviral Vectors

Routine maintenance of cell lines

Mammalian cell culture

shRNA designing

Long PCR

Polymerase chain reaction (PCR)

Gene cloning

DNA fragment isolation from agarose gels

Agarose gel electrophoresis

Large scale preparation of plasmid DNA

Plasmid mini preparation by alkaline lysis

Preparation of ultra competent E. coli

Genomic DNA extraction

Drugs

Lucifearase assay

Cytotoxicity assay

Immunoblotting

Dot-Blotting

Transblotting

SDS-Poly acrylamide gel electrophoresis (PAGE) of proteins

Protein extraction and quantification

RNA extraction/c-DNA synthesis

Lipofectamine method

CaPO4 method

Phosphate buffered saline

Dulbecco’s Modified Eagle Medium

Agarose gel electrophoresis

Transformation

Polymerase Chain Reaction (PCR)

Genomic DNA extraction

Large scale plasmid extraction

Resuspension solution

Transformation buffer

Ultra-competent cells

X-gal

Antibiotics

Luria-Bertani (LB) medium

T-Cell Receptor Mutation Assay

RS-1 Assay

Flow Cytometric analysis of variant erythrocytes

Monoclonal Antibodies against the Human Glycophorin-

MN Blood group typing

T-Cell Receptor Mutation Assay

RS-1 in Cancer samples

RS-1 Assay

Flow Cytometric analysis of variant erythrocytes

Monoclonal Antibodies against the Human Glycophorin

MN Blood group typing

StatisticalAnalysis

Nucleartranslocationofp-Catenin

FlowCytometricanalysis

AnalysisofAP-landNFKBactivatio

Estimationofprostaglandins,PGEzandPGD

Reagents

Reverse Zymograph

Zymography

Reagents

Western Blotting

SOS-PAGE

MTT Assay

AssayofNAD

AktPhosphorylation

ImmunoprecipitationofVEGF

RT-PCR analysis

Separation ofCurcuminoids

Modified Enzyme Linked Immunosorbent Assay

Enzyme Linked ImmunosorbentAssay

Chorioallantoic membrane (CAM) assay

Aortic ring assay

Isolation of endothelial cells from rat aorta

Isolation and culture of HUVECs

Instruments used for the study

MATERIALS

MATERIALANDMETHODS

Reducing powe

Procedur

Reagents

Nitric oxide radical scavenging activity

Procedur

Reagents

Hydroxyl radical scavenging assay

Procedure

Reagent

Superoxide anion scavenging activit

Procedure

Reagent

Total antioxidant activit

Procedur

Reagen

Free radical scavenging activity

In VitroStudies

Chemicals

J. Kalaimathi

Chemopreventive Potential Of Sinapic Acid Against 7 12 Dimethylbenz A Anthracene Induced Hamster Buccal Pouch Carcinogenesis

Single Cell Tes

Electrochemical Measurement

Physical Characterization

Catalyst Preparation

Materials

THE EFFECT OF SEAWATER TOWARDS THE CONDUCTIVITY ENHANCEMENT AND ELECTROCHEMICAL PERFORMANCE ONPt1:Sn1:Mo1/VC NANOCATALYSTS FOR NON-MEMBRANEFUEL

Single Cell Tes

Electrochemical Measurement

Physical Characterizatio

Catalyst Preparation

Material

STRUCTURAL STABILITY AND ELECTROCHEMICAL PERFORMANCE OF W-DOPED PLATINUM ALLOY NANO PARTICLES FOR SEAWATERELECTROLYSIS ON ALCOHOL OXIDATION INNON-MEMBRANE FUEL CELL SYSTEMS

Single Cell Test

Electrochemical Measuremen

Physical Characterization

Catalyst Preparation

Materials

ASSESSMENT OF SEAWATER ELECTROLYSIS ON PLATNIZED TIN AND MAGNESIUM ALLOY NANOPARTICLES FORNON-MEMBRANE POWER SYSTEMS

Single Cell Test

Electrochemical Measuremen

Physical Characterization

Catalyst Preparation

Material

ELECTROGENERATION OF SEAWATER ON ALCOHOL OXIDATION IN AN NON-MEMBRANE POWER SYSTEM

Total alkalinity

Dissolved oxygen and Biological oxygen demand

Total dissolved solids

Multi parameter analyzer

MATERIALS AND METHODS

PHYSICO-CHEMICAL PARAMETERS OF SEAWATER