Assayof xylanase activity

Dinitrosalicylate reagent (DNS)(per liter)

Citrate phosphate buffer

Reagents

Xylanase activity

Evaluation of bioagents against the pathogen

Laboratory screening of antagonists against the test pa

Production of caseinase

Gel documentation and image analysis

Detection of toxR gene

Detection of tlhgene

Extraction of genomic DNA

Detection of V. parahaemolyticusspecies-specific gene

Isolation of V. parahaemolyticuson HiCrome Vibrio

Isolation and identification of V. parahaemolyticus

Gelatinase production

Gelatinase productio

Python Molecular Viewer

RNA Interference

pET30a-β-catenin-FL (Antigen for -catenin antibody)

Protein estimation of sonicate

2 Bioassay

Proliferation Assay

Target binding assay

Cytotoxicity assay

ACK lysis Buffer

lgG-isotyping in the sera of the immunized animals

Genomic DNA extraction

PonceauS stain Instant Blue (Biorad)

Protein loading dye (6X) Tris-Cl (pH 6.8) 300 mM SDS 12% (w/v) Bromophenol blue 0.6% (w/v) Glycerol 60% (v/v) 600 mM β-mercaptoethanol

Stains and Dyes

DNA loading dye

Agarose gel

TAE

For DNA electrophoresis

Stripping Buffer

Leupeptin

Extraction buffer

MTT reagent

For Cytotoxicity assays

Blocking buffer

0.83mL1.5 M Tris-HCl,pH 6.8 50μL10% SDS 50μL10% Ammonium persulfate (APS)8 μLN,N,N′,N′-Tetramethylethylenediamine (TEMED)Resolving gel mix (12%) (20 ml)6.6 mLH2O 8 mL 30% acrylamide:bisacrylamide (29:1) mix 5 mL1.5 M Tris-HCl,pH 8.8 200 μL10% SDS 200 μL10% Ammonium persulfate (APS)8 μLN,N,N′,N′-Tetramethylethylenediamine (TEMED)

Whole cell lysis buffer for yeast (Homogenizing buffer) 50 mM Tris-HCl,pH 7.52 mM EDTA yeastprotease inhibitor cocktail SDS-PAGE 30% Acrylamide solution 29 g acrylamide 1 g bis-acrylamide dissolved in 100 mLH2O. 10% sodium dodecyl sulfate (SDS) 10 g SDS in 100 mLH2O Stacking gel mix (6%)(5 mL)3.4mLH2O 0.63mL 30% acrylamide:bisacrylamide (29:1) mix

Buffers for SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis)