86 Matching Annotations
- Aug 2019
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
University of Delhi
Tags
Annotators
URL
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Materials
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Materials
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Preparation of ZnO/Ag2Onanocomposites
-
- Jun 2019
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Tested individually
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Dominance of regulatory transitional B cellsand lower memory B cells in HBsAgpositive compared to negative newborns
-
Post vaccination enhanced expression of activation marker CD69 and Chemokine receptor CCR5 on memory B cells in HBV positive newborns
-
Post-vaccination declination of Transitional B cell population and increase in memory B cells in HBV positive newborns
-
Increased Chemokine and Toll like receptor on T cells after vaccination in HBV positive newborns.
-
Vaccination improved the Chemokine receptor CCR1, CCR3, CCR9 and Toll like receptor TLR2, TLR4 and TLR9 expression in HBsAgpositive newborns compared to healthy newborns.
-
T cell frequencies(Post vaccination response)
-
Post-vaccination immune responses in newborns
-
IFN γ production by CD8 T cells upon stimulation with PMA and viral peptides
-
Decreased CD3 ζ chain expression on CD8 T cells in HBsAgpositive newborns
-
Phenotypic and Functional Characterization of CD8 T cells in cord blood
-
Cord Blood immune profiles of HBV positive newborns at birth(Cord blood vs. peripheral blood)
-
Pre-vaccination:Lower Chemokine and Toll like receptor expression in HBsAgpositive newborns:
-
T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.
-
Clinical characteristics of the subjects
-
CD107a expression (marker of cytotoxicity)
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Statistical Analysis
-
CFSE proliferation assay
-
CD107a Degranulation Assay
-
Flow cytometric analysis of cytokine production (IFN-γ)by intracellular staining
-
Flow cytometric analysis of intracellular CD3ζ chain expression
-
Cell surface and Intracellular staining and Flow Cytometric Analysis
-
Freezingor storageof cells
-
Cell Counting
-
Separation of Peripheral Blood Mononuclear Cells(PBMCs)from whole blood
-
Virological assessment: Quantitative RT-PCR detection of HBV DNA
-
Serological and Virological Assessment
-
HBV vaccination of the newborn
-
Sample Collection
-
Healthy Controls
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
Shrivastava, Shikha
-
To study the immune status of newborns to hepatitis B positive and negative mothers
-
-
shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
-
Statistical analysis
-
NiSO4. 6H2O stock soloution
-
Nonidet-P40 (NP40) buffer for Ni-NTA pull down
-
Low-pH antibody elution buffer
-
Cytoskeleton extraction buffer (CSE)
-
Semi dry transfer buffer
-
GST elution buffer
-
Ni-NTA elution buffer
-
Lysis buffer for Ni-NTA and glutathione purification
-
1X TBST
-
1X TBS
-
1X PBS
-
Composition of buffers and solutions
-
GST-pull down
-
Surface plasmon resonance (SPR) measurements
-
Expression and purification of recombinant proteins
-
Immunofluorescence studies in SW80 cells for β-catenin under nickel induced condition
-
Luciferase assay in SW480 cells under nickel induced condition
-
Ni-NTA pull down
-
Stripping of Ni-NTA agarose beads
-
Nuclear and cytoplasmic fractionation
-
Large scale purification of BPN in CHO cells from pSecTag2 vector
-
Luciferase reporter assay
-
Immunoprecipitation and western Blotting
-
Immunofluorescence
-
Generation of polyclonal antibodies against GFP, APC and β-catenin
-
RNA Interference
-
Cell transfection
-
Secondary antibodies
-
Primary antibodies
-
Antibodies used in our study
-
Cell culture
-
General reagents and plastic wares
-
Medium and Serum
-
Animals
-
pGEX6P2-BPN (1-993 a.a)
-
pET30a-GFP-FL (GFP antigen)
-
pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)
-
pET30a-β-catenin-FL (Antigen for -catenin antibody)
-
GFP-β-cateninC-C (423-634 a.a) (ARM-C)
-
GFP-β-catenin-C-N (151-423 a.a) (ARM-N)
-
GFP-β-catenin-C (ARM)
-
GFP-β-catenin- C (634 -781 a.a)
-
GFP-β-catenin- a.a
-
GFP-β-catenin T41A mutant
-
Constructs made in the study
-
GFP-β-catenin-FL
Tags
- md-3
- md-15
- md-14-md-5
- md-9
- mt-1-mt-7
- mt-1-mt-4
- md-14-md-9
- md-7
- md-11
- mt-6-mt-2
- mt-6
- mt-3
- mt-1-mt-9
- md-14-md-8
- md-13
- md-17
- md-14-md-4
- md-2
- mt-7
- md-14-md-1
- mt-1
- md-5
- mt-5
- md-8
- mt-1-mt-5
- mt-6-mt-1
- md-16
- md-14-md-7
- md-14-md-3
- mt-1-mt-6
- mt-1-mt-3
- md-14
- mt-1-mt-2
- mt-4
- md-14-md-2
- md-12
- mt-1-mt-1
- mt-1-mt-11
- md-14-md-6
- mt-8
- mt-1-mt-8
- md-10
- mt-2
- md--6
- mt-1-mt-10
- md-4
- md-1
Annotators
URL
-
-
sg.inflibnet.ac.in sg.inflibnet.ac.in
-
National Centre for Cell Science
-
Tripathi Mukesh Mani
-
Novel roles of and#946; catenin and Nup358 in Wnt signaling
-