3 Matching Annotations
- Jun 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Ni-NTA elution buffer
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- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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5 μl of 0.1% TFA was applied to the spots on the SEND array and removed after 30 sec using Whatman paper (care was taken not to touch the spot surface). b. 5 μl of cell lysate sample was spotted on the SEND array and incubated in a humid chamber for 10 min. Removed after 30 min. c. 5 μl of 0.1% TFA was then added and removed after 30 sec. d. 2 μl of 25% ACN in 0.1% TFA was added to the spots and allowed to dry. e. The chip was then placed in the SELDI machine
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Activation of SEND arrays for peptide analysis
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