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  1. Jul 2019
  2. Jun 2019
  3. May 2019
    1. Cell lysis Buffer
    2. Fixative
    3. TAE
    4. Resuspension solution(Solution I)
    5. Polydeoxy (Inosinate-cytidylate) (Poly dI-dC)
    6. Cytoplasmic extractionbuffer (without protease inhibitors)
    7. Fixative : 4% Formaldehyde
    8. Cell lysis buffer(RIPA Buffer)
    9. Phosphate Buffered Saline (PBS)
    10. Ammonium persulfate(APS)
    11. Acrylamide (29:1)
    12. Phenylmethylsulfonyl fluoride (PMSF)
    13. Benzamidine
    14. Aprotinin
    15. Leupeptin
    16. NP-40ComponentsFinal concentrationFor 10 mlNP-4010%1mlH2O9ml
    17. Dithiothreitol (DTT)ComponentsFinal concentrationFor 5 mlDTT1.0M0.7725gH2Oq.s
    18. Ethylenediamine tetraacetic acid (EDTA), pH 8.0ComponentsFinal concentrationFor 500 mlEDTA0.5M93.05gH2Oq.sThe pH is adjusted to 8.0 using 10M NaOH
    19. Ethylene Glycol Tetraacetic acid (EGTA), pH 7.0ComponentsFinal concentrationFor 50 mlEGTA0.1M1.902gH2Oq.sThe pH is adjusted to 7.0 using 10M NaOH
    20. Potassium Chloride (KCl)ComponentsFinal concentrationFor 100 mlKCl2M14.91gH2Oq.s
    21. Sodium Chloride (NaCl)ComponentsFinal concentrationFor 100 mlNaCl5M29.22gH2Oq.s
    22. Potassium Chloride (KCl)
    23. HEPES pH 7.9ComponentsFinal concentrationFor 100 mlHEPES1M23.83gH2Oq.sThe pH wasadjusted to 7.9 using 10M NaOH
    24. Stock solution
    1. Extraction buffer
    2. 10XBinding buffer
    3. Agarose gel
    4. Nuclear lysis buffer (without protease inhibitors
    5. Permeabilisation buffer: 0.2% Triton X100
    6. Stripping buffer
    7. Blocking buffer
    8. TBS-T
    9. Transfer buffer
    10. (f) Running buffer
    11. (e) Stacking polyacrylamide gel
    12. (d) Resolvingpolyacrylamide gel
    13. (c) 6X Protein loading buffer (Lammeli buffer)
    14. (b) Celllysis buffer B(For IB)
    15. Cell lysis bufferA(For IP)
    16. II. For Immunoprecipitation(IP)and Immunoblotting(IB)