585 Matching Annotations
  1. Jul 2019
    1. Description of sampling site
    2. Molecular confirmation of Vibrioby 16S rRNA gene s
    3. Resistance to ampicillin
    4. Citrate utilisation tes
    5. Nitrate reduction tes
    6. Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test
    7. Urease productio
    8. Gelatinase productio
    9. Growth at different temperature
    10. Salt tolerance tes
    11. Voges Proskauer (acetoin production) tes
    12. Indole production
    13. Carbon source utilisation t
    14. Carbohydrate fermentation tes
    15. Amino acids utilisation test (Decarboxylase/dihydrola
    16. Species level identification
    17. Oxidative-Fermentative test
    18. Oxidase tes
    19. Gram staining
    20. Presumptive identification
    21. Isolation of Vibriospecies from water and sediment of Cochin estuary
    22. Sample collection
    23. Analysis of hydrographical parameters
    24. Description of sampling site
    1. Plasmid curing of Vibriofrom Cochin estuary,shrimp farms and seafood
    2. Distribution of antibiotic resistance genes in Vibriofrom Cochin estuary, shrimp farm and seafood
    3. MAR indexingand antibiotic resistance pattern amon
    4. Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood
    5. Antibiotic resistance among Vibriofrom seafood
    6. Antibiotic resistance amongVibriofrom shrimp fa
    7. Antibiotic resistance amongVibriofrom Cochin estu
    8. Antibiotic resistance amongVibrio
  2. sg.inflibnet.ac.in sg.inflibnet.ac.in
    1. Active site identification, metal detection and interaction of Dof domain structure
    2. Superposition of the Dof domain with predicted 3D structure
    3. Validation of the predicted 3D structure
    4. Tertiary structural prediction
    5. Secondary structural prediction
    6. Secondary and tertiary structure prediction of SbDof proteins o
    7. Cis-regulatory elements analysis
    8. Motif analysis
    9. Evolutionary relationships of sorghum, rice and Arabidopsis with respect to Dofgene family
    10. Gene structure prediction
    11. Phylogenetic relationships among Dofproteins of sorghum
    12. Chromosomal locations
    13. In silico prediction of Dof gene family of sorghum
    14. Genome wide identification and in silico characterization of Dof gene family of sorghum
    15. Phylogenetic and motif analysi
    16. In silico characterization of cloned Dof gene
    17. Phylogenetic and motif analysis of sequenced Dof domains
    18. In silico characterization of sequenced Dof domains of cereal
    19. Sequencing of Dof domain and gene
    20. Cloning of Dof genes of sorghum using pBSK vector
    21. Cloning of Dof domain and Dof genes using pGEM-T Easy
    22. Gel elution of PCR products
    23. PCR based cloning, sequencing and in silico characterization of Dof domain and Dofgenes of cereals and millet
    24. Comparative analysis of cereals and millets based on banding patterns generated by Dof domain and Dof genes-specific primers
    25. PCR amplification of Dof gene
    26. PCR amplification of Dofdomain
    27. PCR amplification of Dof domain and Dof genes
    28. Primer designing for PCR amplification of Dof domain and Do
    29. Isolation, purification and quantification of genomic DNA
    30. PCR amplification of Dof domains/genes and studying the polymorphisms generated by different sets of primers for cereals and millets
  3. sg.inflibnet.ac.in sg.inflibnet.ac.in
    1. Active site prediction and docking study
    2. Superposition of predicted structure and template (Dof
    3. Validations
    4. Three dimensional structure prediction, refinements and evaluation of Dof proteins
    5. Cis-regulatory element analysis
    6. Mapping of SbDof genes on sorghum chromosomes and its intron/exon gene structure prediction
    7. In silico prediction of Dof gene family members in S. bicolor (L
    8. Motif identification
    9. Multiple sequence alignment and phylogenetic analysis
    10. In silico analysis of sequenced Dof domain and Dof genes
    11. Reaction resuspension
    12. Post-reaction clean up
    13. Sequencing PCR
    14. Sequencing reaction
    15. Digestion of Plasmid DNA
    16. Minipreparation of plasmid DNA from transformed co
    17. Screening of recombinant E. coli clone
    18. Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)
    19. Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)
    20. Ligation reactions
    21. Dephosphorylation of vector
    22. Restriction digestion
    23. Ligation of eluted PCR product in pBSK vector
    24. Cloning of gel eluted PCR produc
    25. Gel elution of PCR Produ
    26. Scoring of amplification data points and construction of a den
    27. Analysis of PCR amplicons using agarose gel electrophoresis
    28. Cycling condition
    29. PCR reaction set up
    30. Basic requirements for PC
    31. PCR amplification of Dof domain and Dof genes from different
    32. Primer designing for PCR amplification of Dof domain and D
    33. Qualitative analysis of DNA by agarose gel electrophoresi
    34. Spectrophotometric quantification of genomic DNA
    35. DNA purification
    36. DNA extraction procedure
    37. Isolation of genomic DNA by CTAB method
    38. Germination of seeds
    39. Sterilization
    40. Plasmids and bacterial strain
    41. Seed collection of different crops
    42. Glasswares, plasticwares and equipments
    43. Source of chemicals
    1. Estimation of antibody response in rLdADHT+BCG vaccinated hamsters
    2. Estimation of mRNA cytokines in rLdADHT+BCG vaccinated hamsters as well as in control groups
    3. Immunological Responses (DTH, mitogenic and Leishmania-specific cellular responses)
    4. Assessment of parasitic burden in hamsters vaccinated with rLdADHT+BCG and challenged with L. donovan
    5. Statistical analysis
    6. Post-challenge survival
    7. Determination of antileishmanial antibody responses in hamster
    8. RT - PCR
    9. cDNA synthesis and amplification
    10. Formaldehyde gel electrophoresis
    11. RNA isolation
    12. Quantification of mRNA cytokines and inducible NO synthase (iNOS) in hamsters by Real time-PCR
    13. NO production
    14. LTT assay
    15. DTH
    16. Immunological assays
    17. Vaccination schedule and assessment of parasitic burden
    18. 2 Anim
    19. Parasite
    1. The recombinant Th1 stimulatory proteins (rLdADHT, and rLdTPR,) induced lymphoproliferative and NO responses in normal/infected/cured hamst
    2. Solutions used for cytokine assay
    3. Assessment of Cytokine levels- IFN--12/IL-10 in lymphocytes of cured/endemic patients
    4. Nitrite production in macrophages of hamsters
    5. Assessment of Lymphocyte proliferative responses (LTT) in cured/exposed patients and hamsters
    6. Immunological assays
    7. Treatment of L. donovani infected hamsters and isolation of mononuclear cells (lymph node cells)
    8. Patients and isolation of peripheral blood mononuclear cells (PB
    9. Preparation of soluble L. donovani promastigote antigen
    10. Animals
    11. Parasites
    1. rLdTPR was cloned, overexpressed, purified and antibody rais
    2. rLdADHT was cloned, overexpressed, purified and antibody raise
    3. Polyclonal antibody generation
    4. Overexpression and Purification of recombinant protein (rLdTP
    5. Amplification, Cloning and Sequencing
    6. Cloning, Overexpression and purificationof TP
    7. Production of polyclonal antibodies against rLdADHT and We
    8. Purification of recombinant ADHT (rLdADHT)
    9. Recombinant protein Expression
    10. Clone confirmation
    11. Transformation procedure
    12. Transformation in E. coli Rosetta (DE3) cells
    13. Subcloning of ADHT in pET-28a expression vector
    14. Sequencing of ADHT gene
    15. Restriction Digestion of Plasmid DNA
    16. Colony PCR
    17. Preparation of master plate and isolation of plasmid DNA from transformed E. coli (Mini Prep)
    18. Confirmation of positive clone(s)
    19. Transformation
    20. Preparation of chemically competent Escherichia coli using calcium chloride method
    21. Preparation of media for bacterial cell culture
    22. Cloning of gene in pTZ57R/T (T/A cloning) vecto
    23. Elution of amplified gene from the Gel
    24. PCR amplification
    25. Genomic DNA isolatio
    26. Cloning, expression and purification ofADH
  4. Jun 2019
    1. IL-2 Bioassay
    2. IL-2 Bioassay
    3. Statistical Ana(vsis
    4. Western Blot Ana(v.\'is
    5. IL-2 Bioassay
    6. Assay of IFN-yand IL-10 using sandwich ELISA
    7. IL-2 Bioassay
    8. LympllOkine Assays
    9. T cell hybridoma stimulation as.".ays
    10. T cell proliferation assay
    11. Anti-Stm and anti-E coli /gG subclass assay
    12. Total Anti-Stm and anti-E coli antibody assay
    13. Enzyme linked Immunosorbant Assays (ELISA .... )
    14. Injections/or determining MHC-peptide ligand densities
    15. Delayed type hypersensitivi(V (DTH) response
    16. Injections/or determining MHC-peptide ligand densities
    17. Immunisation regimen
    18. Bleeding of mice and Collectiml of Sera
    19. Kinetics of bacterial clearance
    20. Expression of foreign protein in bacteria
    21. Transformation ofStm754 with Ea-cmyc-CiST plasmid
    22. Protein estimation of sonicate