Description of sampling site

Molecular confirmation of Vibrioby 16S rRNA gene s

Resistance to ampicillin

Citrate utilisation tes

Nitrate reduction tes

Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test

Urease productio

Gelatinase productio

Growth at different temperature

Salt tolerance tes

Voges Proskauer (acetoin production) tes

Indole production

Carbon source utilisation t

Carbohydrate fermentation tes

Amino acids utilisation test (Decarboxylase/dihydrola

Species level identification

Oxidative-Fermentative test

Oxidase tes

Gram staining

Presumptive identification

Isolation of Vibriospecies from water and sediment of Cochin estuary

Sample collection

Analysis of hydrographical parameters

Description of sampling site

Plasmid curing of Vibriofrom Cochin estuary,shrimp farms and seafood

Distribution of antibiotic resistance genes in Vibriofrom Cochin estuary, shrimp farm and seafood

MAR indexingand antibiotic resistance pattern amon

Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood

Antibiotic resistance among Vibriofrom seafood

Antibiotic resistance amongVibriofrom shrimp fa

Antibiotic resistance amongVibriofrom Cochin estu

Antibiotic resistance amongVibrio

Active site identification, metal detection and interaction of Dof domain structure

Superposition of the Dof domain with predicted 3D structure

Validation of the predicted 3D structure

Tertiary structural prediction

Secondary structural prediction

Secondary and tertiary structure prediction of SbDof proteins o

Cis-regulatory elements analysis

Motif analysis

Evolutionary relationships of sorghum, rice and Arabidopsis with respect to Dofgene family

Gene structure prediction

Phylogenetic relationships among Dofproteins of sorghum

Chromosomal locations

In silico prediction of Dof gene family of sorghum

Genome wide identification and in silico characterization of Dof gene family of sorghum

Phylogenetic and motif analysi

In silico characterization of cloned Dof gene

Phylogenetic and motif analysis of sequenced Dof domains

In silico characterization of sequenced Dof domains of cereal

Sequencing of Dof domain and gene

Cloning of Dof genes of sorghum using pBSK vector

Cloning of Dof domain and Dof genes using pGEM-T Easy

Gel elution of PCR products

PCR based cloning, sequencing and in silico characterization of Dof domain and Dofgenes of cereals and millet

Comparative analysis of cereals and millets based on banding patterns generated by Dof domain and Dof genes-specific primers

PCR amplification of Dof gene

PCR amplification of Dofdomain

PCR amplification of Dof domain and Dof genes

Primer designing for PCR amplification of Dof domain and Do

Isolation, purification and quantification of genomic DNA

PCR amplification of Dof domains/genes and studying the polymorphisms generated by different sets of primers for cereals and millets

Active site prediction and docking study

Superposition of predicted structure and template (Dof

Validations

Three dimensional structure prediction, refinements and evaluation of Dof proteins

Cis-regulatory element analysis

Mapping of SbDof genes on sorghum chromosomes and its intron/exon gene structure prediction

In silico prediction of Dof gene family members in S. bicolor (L

Motif identification

Multiple sequence alignment and phylogenetic analysis

In silico analysis of sequenced Dof domain and Dof genes

Reaction resuspension

Post-reaction clean up

Sequencing PCR

Sequencing reaction

Digestion of Plasmid DNA

Minipreparation of plasmid DNA from transformed co

Screening of recombinant E. coli clone

Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)

Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)

Ligation reactions

Dephosphorylation of vector

Restriction digestion

Ligation of eluted PCR product in pBSK vector

Cloning of gel eluted PCR produc

Gel elution of PCR Produ

Scoring of amplification data points and construction of a den

Analysis of PCR amplicons using agarose gel electrophoresis

Cycling condition

PCR reaction set up

Basic requirements for PC

PCR amplification of Dof domain and Dof genes from different

Primer designing for PCR amplification of Dof domain and D

Qualitative analysis of DNA by agarose gel electrophoresi

Spectrophotometric quantification of genomic DNA

DNA purification

DNA extraction procedure

Isolation of genomic DNA by CTAB method

Germination of seeds

Sterilization

Plasmids and bacterial strain

Seed collection of different crops

Glasswares, plasticwares and equipments

Source of chemicals

Srivastava, Rashi

Bioinformatics in cancer immunogenomic

Estimation of antibody response in rLdADHT+BCG vaccinated hamsters

Estimation of mRNA cytokines in rLdADHT+BCG vaccinated hamsters as well as in control groups

Immunological Responses (DTH, mitogenic and Leishmania-specific cellular responses)

Assessment of parasitic burden in hamsters vaccinated with rLdADHT+BCG and challenged with L. donovan

Statistical analysis

Post-challenge survival

Determination of antileishmanial antibody responses in hamster

RT - PCR

cDNA synthesis and amplification

Formaldehyde gel electrophoresis

RNA isolation

Quantification of mRNA cytokines and inducible NO synthase (iNOS) in hamsters by Real time-PCR

NO production

LTT assay

DTH

Immunological assays

Vaccination schedule and assessment of parasitic burden

2 Anim

Parasite

The recombinant Th1 stimulatory proteins (rLdADHT, and rLdTPR,) induced lymphoproliferative and NO responses in normal/infected/cured hamst

Solutions used for cytokine assay

Assessment of Cytokine levels- IFN--12/IL-10 in lymphocytes of cured/endemic patients

Nitrite production in macrophages of hamsters

Assessment of Lymphocyte proliferative responses (LTT) in cured/exposed patients and hamsters

Immunological assays

Treatment of L. donovani infected hamsters and isolation of mononuclear cells (lymph node cells)

Patients and isolation of peripheral blood mononuclear cells (PB

Preparation of soluble L. donovani promastigote antigen

Animals

Parasites

rLdTPR was cloned, overexpressed, purified and antibody rais

rLdADHT was cloned, overexpressed, purified and antibody raise

Polyclonal antibody generation

Overexpression and Purification of recombinant protein (rLdTP

Amplification, Cloning and Sequencing

Cloning, Overexpression and purificationof TP

Production of polyclonal antibodies against rLdADHT and We

Purification of recombinant ADHT (rLdADHT)

Recombinant protein Expression

Clone confirmation

Transformation procedure

Transformation in E. coli Rosetta (DE3) cells

Subcloning of ADHT in pET-28a expression vector

Sequencing of ADHT gene

Restriction Digestion of Plasmid DNA

Colony PCR

Preparation of master plate and isolation of plasmid DNA from transformed E. coli (Mini Prep)

Confirmation of positive clone(s)

Transformation

Preparation of chemically competent Escherichia coli using calcium chloride method

Preparation of media for bacterial cell culture

Cloning of gene in pTZ57R/T (T/A cloning) vecto

Elution of amplified gene from the Gel

PCR amplification

Genomic DNA isolatio

Cloning, expression and purification ofADH

Results of Python Molecular Viewer

Evaluation of QSAR models

2D QSAR

Results of molecular docking

Python Molecular Viewer

PubChem Compound Database Screening

2D QSAR

Cygwin

Molecular Docking

Inhibitors Dataset

Active site analysis

Receptor X-ray structure

Molecular Docking

Inhibitors Dataset

Receptor X-ray Structure

IL-2 Bioassay

IL-2 Bioassay

Statistical Ana(vsis

Western Blot Ana(v.\'is

IL-2 Bioassay

Assay of IFN-yand IL-10 using sandwich ELISA

IL-2 Bioassay

LympllOkine Assays

T cell hybridoma stimulation as.".ays

T cell proliferation assay

Anti-Stm and anti-E coli /gG subclass assay

Total Anti-Stm and anti-E coli antibody assay

Enzyme linked Immunosorbant Assays (ELISA .... )

Injections/or determining MHC-peptide ligand densities

Delayed type hypersensitivi(V (DTH) response

Injections/or determining MHC-peptide ligand densities

Immunisation regimen

Bleeding of mice and Collectiml of Sera

Kinetics of bacterial clearance

Expression of foreign protein in bacteria

Transformation ofStm754 with Ea-cmyc-CiST plasmid

Protein estimation of sonicate