this paper described some hit compounds that interfere with the assay components.
some compounds react with thiol, and their SAR is reported.
Journal of the American Chemical Society.
however, there was noevidence of covalent bonding to the protein.
this result is similar to CNIBR result, thus it is important to assess the cysteine reactivity before any MedChem efforts.
SDS-PAGE disrupts noncovalent interactions and is used to determine covalent binding.
SDS-PAGE is used to identify covalent binder with the help of click chemistry
covalent fragment screening