These neurons express multiple itch receptors including MrgprA3, MrgprC11, and histamine receptor 1, and correspondingly respond to multiple pruritogens. Deletion of MrgprA3+ sensory neurons significantly inhibits itch behavior not only in acute itch conditions14, but also in chronic itch conditions13,15, demonstrating that this neuronal population is crucial for mediating itch sensation. However, whether the chronic itch response requires the activation of Mrgprs in MrgprA3+ neurons is unknown.