expression is tightly regulated
cumate inducible
4 Matching Annotations
- Nov 2020
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journals.asm.org journals.asm.org
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- Apr 2020
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www-ncbi-nlm-nih-gov.ezproxy.rice.edu www-ncbi-nlm-nih-gov.ezproxy.rice.edu
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In this paper, we designed a system that could fully repress lycopene production in the absence of an inducer and produce visible lycopene within two hours of induction. We engineered Lac, Ara, and T7 systems to be up to 10 times more repressible, but these improved systems could still not fully repress lycopene. Translational modifications proved much more effective in controlling lycopene. By decreasing the strength of the ribosomal binding sites on the crtEBI genes, we enabled full repression of lycopene
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- Aug 2019
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www-sciencedirect-com.ezproxy.rice.edu www-sciencedirect-com.ezproxy.rice.edu
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While these techniques offer good repression, they exhibit leakiness that precludes the gene of interest from being completely turned off
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ami-journals-onlinelibrary-wiley-com.ezproxy.rice.edu ami-journals-onlinelibrary-wiley-com.ezproxy.rice.edu
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Clearly, premature removal of the cI cassette in the absence of an inducer can limit environmental applications of this type of biosensor
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