24 Matching Annotations
- Oct 2023
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www-nature-com.ezproxy.rice.edu www-nature-com.ezproxy.rice.edu
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sRNAs that repress transcription have been engineered to create orthogonal and composable regulators that can be used to construct RNA-only transcriptional networks
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- Dec 2022
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www.thermofisher.com www.thermofisher.com
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Diagram of immunoprecipitation (IP) using either pre-immobilized or free antibodies.
Immunoprecipitation is a technique for the isolation of protein or a complex (protein-protein interactions) Sample is combined with a specific antibody for the epitope of interest. The antibody-protein complex is removed and analysed.
- Molecules from biological sample (lysed) +incubated with antibodies (free or mounted onto support (like agarose bead, magnetic bead))
- protein A or G coupled beads added.
- Centrifuged
- Results in Beads with protein A/G bound to antibody-POI complex.
- Well separated in this way, differentially based on sedimentation coefficient.
Co-immunoprecipitation (can isolate one type of protein in its complex)
Isolate POI(s) Good with low conc. of POI Protein interactions Unknown proteins Determine if protein is actually being expressed in a given tissue.
Western blot is carried out to analyse the output.
Vary salt and detergent levels to preserve or destroy protein interactions.
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bio.libretexts.org bio.libretexts.org
- Apr 2022
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microbiology.okstate.edu microbiology.okstate.edu
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GenExpDB is the world’s largest repository for E. coli gene expression data. This site is a widely used public resource for gene expression analysis.
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- May 2021
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Local file Local file
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The basal level of T7-dependent tran-scription in this strain can be reduced by constitutive produc-tion of T7 lyzozyme, a natural inhibitor of T7 RNAP, usingplasmids pLysS and pLysE
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- Nov 2020
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journals.asm.org journals.asm.org
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expression is tightly regulated
cumate inducible
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- Sep 2020
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www-embopress-org.ezproxy.rice.edu www-embopress-org.ezproxy.rice.edu
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genetic devices have been designed for suppressing leaky basal expression levels through the engineering of super‐repressors (Ruegg et al, 2018), exploitation of antisense RNAs (O'Connor & Timmis, 1987), or physical decoupling of regulatory elements along with conditional proteolysis
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- Feb 2020
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www.nature.com www.nature.com
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the leakiness and inducibility of the ligand-inducible LacI repressor mutants have been quantified and classified
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URL
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aem.asm.org aem.asm.org
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Consequently, the plasmid donor cells do not express gfp
Does the leaky expression not hinder the detecting and quantification of transconjugants in epifluorescence microscopy?
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- Oct 2019
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journals.plos.org journals.plos.org
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We have demonstrated that small RNAs can tightly repress their target genes when their synthesis rate is smaller than some threshold, but have little or no effect when the synthesis rate is much larger than that threshold
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www.sciencedirect.com www.sciencedirect.com
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In particular, small RNAs are shown to establish a threshold for the expression of their target, providing safety mechanism against random fluctuations and transient signals. The threshold level is set by the transcription rate of the small RNA and can thus be modulated dynamically to reflect changing environmental conditions.
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www.annualreviews.org www.annualreviews.org
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in the absence of tamoxifen, it exhibits some activity
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strong promoters capable of driving expression of microbial opsins or fluorescent proteins in specific populations can exhibit leaky expression elsewhere. This low-level leak may be virtually undetectable as light responsiveness or fluorescence but can be a serious issue when expressing Cre recombinase.
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www.embopress.org www.embopress.org
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it can be useful for reducing leaky expression of toxic proteins
antisense transcription
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aem.asm.org aem.asm.org
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We report the construction of a family of vectors that contain a reengineered lacIq-lac promoter-operator complex in which cloned genes are strongly repressed in the absence of inducer.
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- Aug 2019
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www-sciencedirect-com.ezproxy.rice.edu www-sciencedirect-com.ezproxy.rice.edu
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While these techniques offer good repression, they exhibit leakiness that precludes the gene of interest from being completely turned off
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- Jul 2019
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www.plantcell.org www.plantcell.org
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Check out the peer review report for this article: http://www.plantcell.org/content/plantcell/suppl/2019/07/12/tpc.18.00785.DC2/tpc18.00785.PeerReviewReport.pdf
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- Oct 2018
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www.nature.com www.nature.com
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Massive mining of publicly available RNA-seq data from human and mouse
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- Jul 2018
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www.sciencedirect.com www.sciencedirect.com
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repeat expansion at IIL1 leads to increased accumulation of 24-nt siRNAs in a temperature-dependent manner that correlates with the iil phenotype. We show that DCL3 and other components of the RNA-dependent DNA methylation (RdDM) pathway are essential for this siRNA-directed epigenetic gene silencing
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- Nov 2017
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Robust enumeration of cell subsets from tissue expression profiles
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Gene Set Enrichment Analysis Made Simple
using aggregate t or chi^2 statistic to test if a set of genes is on aggregate differentially expressed
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- Sep 2017
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www.biorxiv.org www.biorxiv.org
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Massive Mining of Publicly Available RNA-seq Data from Human and Mouse
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- Jul 2017
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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The ISMARA client
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genome.cshlp.org genome.cshlp.org
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ISMARA: automated modeling of genomic signals as a democracy of regulatory motifs
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