18 Matching Annotations
  1. Jul 2019
    1. Sequence analysis
    2. DNA sequencing of the 18S rDNA fragment
    3. Purification of PCR product
    4. Analysis of internal transcribed spacer region
    5. RAPDand SSRscoring and data analysis
    6. PCR amplification
    7. Running of gel and visualization of DNA
    8. Determination of the yield
    9. Agarose gel electrophoresis
    10. Qualitative and quantitative estimation of DNA
    11. Determination of the yield
    12. Procedure for DNA isolation
    13. Reagents required for fungal DNA isolationand p
  2. Jun 2019
  3. May 2019
    1. Lysis Buffer: 0.1% Triton X-100/0.1 M Tris-HCl (pH 8.0). 450 ml distilled water 50 ml 1M Tris-HCl (pH 8.0) 0.5 ml Triton X-100 detergent • 100X Mg++ solution: 0.1 M magnesium chloride 4.5 M 2-mercaptoethanol Stored at 4°C. • 0.1 M sodium phosphate (pH 7.5)41 ml 0.2 M Na2HPO4 9 ml 0.2 M Na H2PO4 50 ml distilled water • 4 mg/ml ONPG (o-nitrophenyl-β-D-galactopyranoside) in 0.1 M sodium phosphate (pH 7.5) containing 2 mM β-mercaptoethanol, Stored at –20°C. • 0.1 mg/ml β-gal standard: 0.1 mg/ml β-gal in 0.1 M sodium phosphate (pH 7.5) containing 2 mM 2-mercaptoethanol Stored at 4°C. • 1 M sodium carbonate in water
    2. Solutions: