3 Matching Annotations
  1. Aug 2021
    1. Skin Extract Preparation

      Alarm substance was collected by making shallow lesions on the skin (7 to 10 on each side) using a sharp razor (Sharpoint, 22.5o stab), such that drawing of blood was minimal. Fish were then immersed in 2 ml of 20 mM Tris-Cl (pH 8.0) for 20-30 seconds. The process was repeated for 7 fish and extract collected into the same volume. The 2 ml crude extract was then centrifuged at 13.2k rpm and filtered. All steps were performed at 4 ̊C. 1 unit of extract was defined as the minimal amount of skin extract that must be delivered into the test tank to elicit freezing. A typical extraction yielded 20 units.

      Sloughing was performed by mechanical shaking, either by inverting a 2 ml eppendorf tube with a euthanized fish without lesions or cuts in 2 ml of 20 mM Tris-Cl (pH 8.0), rapidly many times or on an orbital shaker for 1’.

      For estimation of chondroitin concentration extraction was performed in 100 mM CH3COONH4 as described.

  2. Jul 2021
    1. 此研究的研究对象是健康足月胎儿,最终可用样本量为19,观察他们对连续24次震动刺激的反应,当对连续的4个刺激不产生反应时视为习惯化。每个研究对象进行3次实验,第1次实验结束的10分钟后和24小时后重复测试,以观察短期记忆和长期记忆能力。

  3. Jun 2021