动物模型中的慢性压力导致的同种异体超负荷导致海马和脑前额叶外皮神经元萎缩，大脑中与记忆、选择性注意力和执行功能相关的区域萎缩，并导致杏仁核中神经元肥大，杏仁核是一个与恐惧、焦虑以及攻击性相关的大脑区域。Iv).因此，学习、记忆和做决定的能力可能会受到长期压力的影响，并可能伴随着焦虑和攻击性程度的增加。

《Journal of Neuroscience Methods》在神经科学领域中为Q4，2020年的影响因子为2.39

Skin Extract Preparation

Alarm substance was collected by making shallow lesions on the skin (7 to 10 on each side) using a sharp razor (Sharpoint, 22.5o stab), such that drawing of blood was minimal. Fish were then immersed in 2 ml of 20 mM Tris-Cl (pH 8.0) for 20-30 seconds. The process was repeated for 7 fish and extract collected into the same volume. The 2 ml crude extract was then centrifuged at 13.2k rpm and filtered. All steps were performed at 4 ̊C. 1 unit of extract was defined as the minimal amount of skin extract that must be delivered into the test tank to elicit freezing. A typical extraction yielded 20 units.

Sloughing was performed by mechanical shaking, either by inverting a 2 ml eppendorf tube with a euthanized fish without lesions or cuts in 2 ml of 20 mM Tris-Cl (pH 8.0), rapidly many times or on an orbital shaker for 1’.

For estimation of chondroitin concentration extraction was performed in 100 mM CH3COONH4 as described.

此研究的研究对象是健康足月胎儿，最终可用样本量为19，观察他们对连续24次震动刺激的反应，当对连续的4个刺激不产生反应时视为习惯化。每个研究对象进行3次实验，第1次实验结束的10分钟后和24小时后重复测试，以观察短期记忆和长期记忆能力。

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