His most well-known work was the Thesaurus graecae linguae, which was printed in five volumes. The basis of Greek lexicology, no thesaurus would rival that of Estienne's for three hundred years.

The Thesaurus Linguae Graecae (TLG) is a research center at the University of California, Irvine. The TLG was founded in 1972 by Marianne McDonald (a graduate student at the time and now a professor of theater and classics at the University of California, San Diego) with the goal to create a comprehensive digital collection of all surviving texts written in Greek from antiquity to the present era.