24 Matching Annotations
- May 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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15 mM CaCl2.2H2O 250 mM KCl 55 mM MnCl2.4H2O pH was adjusted to 6.7 with 1 N KOH. MnCl2needsto beaddedseparately,drop by drop with stirring, tothe buffer. PIPES goes into solutionwhenpH is greater than 6.7. The solution, after pH adjustment to 6.7 was filter-sterilized and stored at -20ºC.Reagents for yeast transformation:1 M Lithium acetate (LiOAc)50% Polyethylene glycol10 mg/ml Carrier DNADimethylsulfoxide (DMSO)
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INOUE transformation buffer:For bacterial DH5α ultra-competent cells preparation10 mM PIPES (free acid)
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Binding Buffer (10X)
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Blocking buffer: 2% BSA
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Permeabilization buffer: 0.2% Triton X100
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Fixative : 4% Formaldehyde
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Running Buffer
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Ethylene Glycol Tetraacetic acid (EGTA), pH 7.0ComponentsFinal concentrationFor 50 mlEGTA0.1M1.902gH2Oq.sThe pH is adjusted to 7.0 using 10M NaOH
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Extraction buffer
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10XBinding buffer
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Agarose gel
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Nuclear lysis buffer (without protease inhibitors
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Permeabilisation buffer: 0.2% Triton X100
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Stripping buffer
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Blocking buffer
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TBS-T
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Transfer buffer
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(f) Running buffer
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(e) Stacking polyacrylamide gel
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(d) Resolvingpolyacrylamide gel
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(c) 6X Protein loading buffer (Lammeli buffer)
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(b) Celllysis buffer B(For IB)
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Cell lysis bufferA(For IP)
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(b) Tris Buffered Saline (TBS)
Tags
- Mt-4-Mt-1-Mt-4-Mt-2-d
- Mt-4-Mt-1-Mt-8-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-6-d
- Mt-4-Mt-1-Mt-3-Mt-2-d
- Mt-4-Mt-1-Mt-7-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-9-d
- Mt-4-Mt-1-Mt-5-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-3-d
- Mt-4-Mt-1-Mt-2-Mt-8-d
- Mt-4-Mt-1-Mt-2-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-1-d
- Mt-4-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-5-d
- Mt-4-Mt-1-Mt-2-Mt-4-d
- Mt-4-Mt-1-Mt-2-Mt-7-d
Annotators
URL
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