Cytotoxicity assay by Sulphorhodamine B (SRB) me

Germination of N. sativaseeds

Activation of TLC plate

Tests for Alkaloids

Germination of N.sativaseeds

Testing of bioformulations

Sequence analysis

DNA sequencing of the 18S rDNA fragment

Purification of PCR product

Analysis of internal transcribed spacer region

RAPDand SSRscoring and data analysis

PCR amplification

Running of gel and visualization of DNA

Determination of the yield

Agarose gel electrophoresis

Qualitative and quantitative estimation of DNA

Determination of the yield

Procedure for DNA isolation

Reagents required for fungal DNA isolationand p

DNA isolation of Trichodermaisolate

Genetic variability analysis through RAPD and SSR

Photography, evaluation and documentation

Procedurefor SDS-PAGE

Materialsrequired for SDS-PAGE

Protein profiling of bioagent through SDS-PAGE

Assayof xylanase activity

Dinitrosalicylate reagent (DNS)(per liter)

Sterilization

Effect of temperature on growth of bioagent

Identificationof bioagent

Isolation and purification of Trichoderma sp.

Sterilization of media and distilled water

Source of chemicals

Prevalence of extracellular virulence factors in Vibriofrom shrimp farm

Gel documentation and image analysis

Screening for virulence genes in V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood

Bacterial strains used

DNAisolation

Detection of virulence genes tdhand trhby multiplex

Detection of type III secretion system genes

Production of Lipase

MAR indexingand antibiotic resistance pattern amon

Antibiotic resistance amongVibriofrom shrimp farm

Detection of blaTEMgene

MAR indexing

Antibiotic resistance amongVibriofrom Cochin estu

Species level identification and distribution of Vibriospecies in Cochin estuary

Gel documentation and image analysis

Detection of toxR gene

Detection of tlhgene

Extraction of genomic DNA

Detection of V. parahaemolyticusspecies-specific gene

PCR amplification of 16S rRNA gene

Carbohydrate fermentation test

Oxidase test

Analysis of hydrographical parameters

Carbohydrate fermentation tes

Oxidase tes

Analysis of hydrographical parameters

Active site identification, metal detection and interaction of Dof domain structure

Superposition of the Dof domain with predicted 3D structure

Validation of the predicted 3D structure

Tertiary structural prediction

Chromosomal locations

Phylogenetic and motif analysi

In silico characterization of cloned Dof gene

Phylogenetic and motif analysis of sequenced Dof domains

In silico characterization of sequenced Dof domains of cereal

Sequencing of Dof domain and gene

Cloning of Dof genes of sorghum using pBSK vector

Cloning of Dof domain and Dof genes using pGEM-T Easy

Gel elution of PCR products

PCR based cloning, sequencing and in silico characterization of Dof domain and Dofgenes of cereals and millet

PCR amplification of Dof gene

Primer designing for PCR amplification of Dof domain and Do

Active site prediction and docking study

Superposition of predicted structure and template (Dof

Validations

Three dimensional structure prediction, refinements and evaluation of Dof proteins

Cis-regulatory element analysis

Mapping of SbDof genes on sorghum chromosomes and its intron/exon gene structure prediction

In silico prediction of Dof gene family members in S. bicolor (L

Motif identification

Multiple sequence alignment and phylogenetic analysis

In silico analysis of sequenced Dof domain and Dof genes

Reaction resuspension

Post-reaction clean up

Sequencing PCR

Sequencing reaction

Digestion of Plasmid DNA

Minipreparation of plasmid DNA from transformed co

Screening of recombinant E. coli clone

Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)

Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)

Ligation reactions

Dephosphorylation of vector

Restriction digestion

Ligation of eluted PCR product in pBSK vector

Cloning of gel eluted PCR produc

Gel elution of PCR Produ

Scoring of amplification data points and construction of a den

Analysis of PCR amplicons using agarose gel electrophoresis

Cycling condition

PCR reaction set up

Basic requirements for PC

PCR amplification of Dof domain and Dof genes from different

Primer designing for PCR amplification of Dof domain and D

Qualitative analysis of DNA by agarose gel electrophoresi

Spectrophotometric quantification of genomic DNA

DNA purification

DNA extraction procedure

Isolation of genomic DNA by CTAB method

Glasswares, plasticwares and equipments

Immunological Responses (DTH, mitogenic and Leishmania-specific cellular responses)

Formaldehyde gel electrophoresis

LTT assay

2 Anim

Nitrite production in macrophages of hamsters

Animals

rLdTPR was cloned, overexpressed, purified and antibody rais

Overexpression and Purification of recombinant protein (rLdTP

Clone confirmation

Colony PCR

Transformation

PCR amplification

2D QSAR

Active site analysis

Inhibitors Dataset

Assay for Lipid peroxidase(LPO)

Cytomorphological analysis

Growth kinetic studies

Reducing power assay

TEM-EDAX analysis

Qualitative analysis of compounds

The table 6.1 gives the mixing probabilities and the associated parametricvalues fork(number of components) = 2,3, and 4. It may be noted thatthe Log likelihood value is smaller fork= 4 (the results fork= 5 , 6 etc.are not better than that fork= 4 and hence are not given here). The fourcomponents Poisson Mixture model is given in table 6.2. It may be notedthat 58% of wards may have higher incidence/relative risk and the remainingwards have lesser/lower incidence for the Cancer disease. We computed theposterior probability for each component for each ward (see table 6.3). Eachward is assigned to a particular component so that the posterior probability islarger. These results are also given in table 6.3 Finally we present Choroplethmaps based on those results

The Posterior Probability of Mixing Dis-tribution

Algorithm

EM Procedure

Poisson Mixture

Data Sources

Poisson Mixtures Distribution

Poisson Model

Haemolymph protein profile

Helicoverpa armiger

Spodoptera litura

Gut enzyme profile

Haemolymph protein profiling

Lactate dehydrogenase

VS preparation

Utilization of VS by the prey

Influence of prey on VS yield

Quantification of protein of VS yielded in the prey deprivation

Survival (SR) and venom milking rate (VMR

Venomous saliva optimization

Statistical analysi

Quantification of VS for protein

Venomous saliva utilization

Prey type

Starvation and collection method

Venomous saliva optimization

Insects Collection

VENOMOUS SALIVA: OPTIMIZATION AND UTILIZATION

Mandibular stylet

Head and stylet preparation

Micronuclei test

Malic dehydrogenase

Alkaline Phosphatase

Glycogen content

Analysis of Physico-chemical parameters of the water sampled

Increments in biomass:

Residual nutrients

Chlorophyll content in waters:

Growth maximum values

Rates of growth (OD678/day

Growth

Experimental Set up

BIOMASS PRODUCTION: ROLE OF N-P CONCENTRATION AND RATIOS

Soil pH and electrical conductivity (EC)

Experiment 2: Assessing the impacts of elevated temperature and N levels on yield and nutrient uptake in rice

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Experimental Design and Treatments

Temperature Gradient Tunnels (TGT)

Yield and its component traits:

Differences among cultivars for yield, biomass and HI

Kernel hardness

SDS-sedimentation test

Estimation of total N% of wheat grainsand straw

End use Quality parameters

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Laboratory observations

Days to physiological maturity

Static and shaken condition

Screening for proteolytic activity

Vaccination improved the Chemokine receptor CCR1, CCR3, CCR9 and Toll like receptor TLR2, TLR4 and TLR9 expression in HBsAgpositive newborns compared to healthy newborns.

IFN γ production by CD8 T cells upon stimulation with PMA and viral peptides