a kanamycin resistance gene known to be expressed in both Gram-negative and Gram-positive bacteria

RSF1010 is maintained at a copy-number of about 10-12 per chromosome in E coli, P. aeruginosa and S. enterica sv.Typhimurium (Frey and Bagdasarian, 1989)

This work provides CasTn as a new method for host-independent, programmable, targeted DNA insertions to expand the genomic engineering toolbox.

The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica

Among the fully sequenced BHR plasmids, most are classified as the well-known incompatibility groups, such as IncP-1 (41 plasmids), IncW (5 plasmids), and IncU (4 plasmids), based on the backbone genes

The host range for three of the plasmids (pBF1, pB7, and pB9) was further investigated by the GFP method described above

Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism

RP4 has been shown to exhibit a very broad host range and has been demonstrated to efficiently transfer in activated sludge (22, 40), chicken manure (24), and the rhizospheres of various plants (38, 39).