20 Matching Annotations
  1. May 2023
  2. Mar 2023
    1. ed away by theintense enjoyment he found in these pleasant fancies, he sethimself forthwith to put his scheme into execution

      This is what I like about Don Quixote. He think of what he wants to do first and execute it, instead of what he can do, because I know that if I only think of what I can do in the beginning, then I will achieve nothing.

    2. In short, his wits being quite gone, he hit upon the strang-est notion that ever madman in this world hit upon, and thatwas that he fancied it was right and requisite, as well for thesupport of his own honour as for the service of his country,that he should make a knight-errant of himself,

      Mostly, ambition is perceived as mad, but being mad is not always a bad thing.

  3. May 2019
    1. 5 μl of 0.1% TFA was applied to the spots on the SEND array and removed after 30 sec using Whatman paper (care was taken not to touch the spot surface). b. 5 μl of cell lysate sample was spotted on the SEND array and incubated in a humid chamber for 10 min. Removed after 30 min. c. 5 μl of 0.1% TFA was then added and removed after 30 sec. d. 2 μl of 25% ACN in 0.1% TFA was added to the spots and allowed to dry. e. The chip was then placed in the SELDI machine
    2. DNA fragments to be used for ligation were eluted from the agarose gel, after the electrophoresis. The gel piece containing the desired band of DNA was sliced out and the DNA was purified from the gel using the purification kits available for this purpose. The efficiency of elution was determined by checking a small aliquot of DNA sample on the gel