Effect of temperature on xylanase activity

Effect of pH on xylanase activity

Effect of various carbon sourceson xylanase activity

Assayof xylanase activity

Dinitrosalicylate reagent (DNS)(per liter)

Citrate phosphate buffer

Reagents

Xylanase activity

Harvesting of cultures

Enzyme production (EP) medium

Inoculum preparation

Sporulation medium used for Trichodermasp

Maintenance of Trichodermasp. culture

Sterilization

Materials for xylanase induction

Induction of xylanase from Trichodermasp

Detection of hemolytic activity

Statistical analysis

Urease production

Urease productio

pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)

Transformation ofStm754 with Ea-cmyc-CiST plasmid

IFN Biossay

Expression of costimulatory molecules on the vaccines

Phenotypic analysis by Flow cytometry

Polymerase Chain Reaction (PCR)

Most chemicals were obtained from commercial sources. The sources for some of the fine chemicals used in this study are given below. Most of the chemicals such as amino acids, antibiotics, sugars, IPTG, ONPG and X-gal were obtained from Sigma Chemical Co. The media components for the growth of bacteria were routinely from Himedia. The materials used in the recombinant DNA experiments such as restriction endonucleases, T4 DNA ligase, DNA polymerases for PCR amplification and DNA size markers were obtained from companies including New England Biolabs and Fermentas. Quiagen or HiPura Kits used for plasmid isolation, purification of DNA fragments. The oligonucleotide primers used in this study were mainly synthesized on order by Ocimum Biosolutions or MWG Biotech Pvt. Ltd

Chemicals

Inoue buffer

For preparation of Ultra competent cells

Aprotinin

Stripping buffer