13 Matching Annotations
  1. Dec 2020
    1. Antibiotics, according to strain sensitivity, were used at the following concentrations: tetracycline 2 μg/ml for Rm1021, BM7, BM299 and BM300, 4 μg/ml for LMG18864, 10 μg/ml for other Sinorhizobium strains and for E. coli; streptomycin 600 μg/ml and kanamycin 200 μg/ml were used for Rm1021; kanamycin for E. coli S17.1 was used at 40 μg/ml; gentamicin was used at 20 μg/ml for all strains
  2. Nov 2020
    1. Antibiotic concentrations used for plasmid selection in V. natriegens: ampicillin–carbenicillin 100 µg ml−1, kanamycin 75 µg ml−1, chloramphenicol 5 µg ml−1, spectinomycin 100 µg ml−1. E. coli experiments were performed in standard LB and M9 media.
  3. Jan 2020
    1. Vmax has a low-level natural resistance to kanamycin but is more susceptible to other antibiotics. When selecting on Kan plates, Vmax with KanR will simply grow faster than Vmax without KanR. Larger colonies=KanR. Other antibiotics select against Vmax but must have a dosage change compared to E. coli.
    1. After all, the rise in antibiotic resistance doesn’t mean that we shouldn’t have invented antibiotics at all.

      It does mean though, that we should try to forsee unintended consequences and not dismiss skeptics without judging the merit of their skepticism with sound scientific discussions. It is famed that Alexander Fleming who discovered the first antibiotic - penicillin, mentioned the observation of resistant bacteria and advised caution in antibiotic usage. This caution went unheeded in the subsequent golden era of antibiotics where all alternatives like phage therapy were shelved from research and development. This is the real lesson we should learn

  4. Oct 2019
    1. indicate that even low concentrations of just a single type of antibiotic leads to resistance to multiple classes of antibiotics.

      I doubt this claim, do you mean they are correlated?

    1. Overall, the low contamination level of antibiotics and high absolute abundance of ARGs indicated that ARGs previously introduced to an environment can self-amplify and persist in the environment
    2. suggesting that STPs have become an important source of contamination for their receiving rivers

      Read these references: Saying antibiotic resistance is proliferating in wastewater treatment plants

    3. Additionally, the concentrations of antibiotics and the abundance of ARGs might increase after the sewage treatment process.

      Wow, that is a strong and counter-intuitive claim. Is this rigorously determined?

    1. wastewater treatment plants are hotspots for the release of antibiotics, and transmission of diverse antibiotic resistance bacteria (ARB) as well as ARGs into various environments

      That's a strong claim, not backed by strong evidence

    2. Recent findings have indicated that most ARGs could not be efficiently removed by traditional anaerobic digestion treatment
  5. Aug 2019
    1. diagnosis of antibiotic interference in the major biosynthetic pathways of bacteria: the biosynthesis of DNA by the yorB promoter, of RNA by the yvgS promoter, of proteins by the yheI promoter, of the cell wall by the ypuA promoter, and of fatty acids by the fabHB promoter.
  6. Jun 2019
    1. introduction of Cu to agricultural soil selects for Cu resistance, but also indirectly selects for antibiotic resistance in the Cu‐resistant bacteria