DNA sequencing of the 18S rDNA fragment

Purification of PCR product

Analysis of internal transcribed spacer region

RAPDand SSRscoring and data analysis

PCR amplification

Running of gel and visualization of DNA

Determination of the yield

Agarose gel electrophoresis

Qualitative and quantitative estimation of DNA

Determination of the yield

Procedure for DNA isolation

Reagents required for fungal DNA isolationand p

DNA isolation of Trichodermaisolate

Photography, evaluation and documentation

Procedurefor SDS-PAGE

Materialsrequired for SDS-PAGE

Protein profiling of bioagent through SDS-PAGE

Biochemical analysis (Protein estimation)

Protein estimation through Kjeldahl method

Dinitrosalicylate reagent (DNS)(per liter)

Citrate phosphate buffer

Reagents

Materials for xylanase induction

Evaluation of bioagents against the pathogen

Effect of different media on growth of bioagentTrichoderma

Identificationof bioagent

Isolation and purification of pathogen, Fusarium udum

Sterilization of glasswares

Experimental site

Prevalence of extracellular virulence factors in Vibriofrom Cochin estuary

Statistical analysis

Detection of hemolytic activity

Production of caseinase

Production of phosphatase

Bacterial strains used

Production of chitinase

Production of DNas

Production of gelatinase

Production of Lipase

Production of amylase

Bacterial strains used

Screening of Vibriostrains for extracellular enzymes

Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood

Antibiotic resistance among Vibriofrom seafood

Antibiotic resistance amongVibriofrom shrimp farm

Antibiotic resistance amongVibriofrom Cochin estu

Antibiotic resistance amongVibrio

DNA isolation

Antibiotic sensitivity tes

Antibiotic resistance amongVibrio

Environmental parameters

Extraction of genomic DNA

Isolation of V. parahaemolyticuson HiCrome Vibrio

DNA isolatio

Amino acids utilisation test (Decarboxylase/dihydrol

Gram staining

Description of sampling site

Amino acids utilisation test (Decarboxylase/dihydrola

Gram staining

Description of sampling site

Validation of the predicted 3D structure

Secondary structural prediction

In silico prediction of Dof gene family of sorghum

Phylogenetic and motif analysi

Phylogenetic and motif analysis of sequenced Dof domains

Gel elution of PCR products

PCR amplification of Dofdomain

Isolation, purification and quantification of genomic DNA

PCR amplification of Dof domains/genes and studying the polymorphisms generated by different sets of primers for cereals and millets

Validations

Mapping of SbDof genes on sorghum chromosomes and its intron/exon gene structure prediction

Sequencing PCR

Minipreparation of plasmid DNA from transformed co

Restriction digestion

Basic requirements for PC

DNA extraction procedure

Germination of seeds

Source of chemicals

Assessment of parasitic burden in hamsters vaccinated with rLdADHT+BCG and challenged with L. donovan

RNA isolation

DTH

Parasite

The recombinant Th1 stimulatory proteins (rLdADHT, and rLdTPR,) induced lymphoproliferative and NO responses in normal/infected/cured hamst

Solutions used for cytokine assay

Assessment of Lymphocyte proliferative responses (LTT) in cured/exposed patients and hamsters

Parasites

rLdADHT was cloned, overexpressed, purified and antibody raise

Amplification, Cloning and Sequencing

Transformation procedure

Preparation of master plate and isolation of plasmid DNA from transformed E. coli (Mini Prep)

Preparation of chemically competent Escherichia coli using calcium chloride method

Genomic DNA isolatio

Cloning, expression and purification ofADH

Results of molecular docking

Receptor X-ray structure

Receptor X-ray Structure

Estimation of Total protein

MTT [(4, 5–dimethylthiazol–2–yl)–2, 5-diphenyl tetrazolium Bromide] assay

Kirby-Bauer Disk diffusion method

DPPH free radical quenching activity

UV–visible spectral analysis

Collection and preparation of extracts

The table 6.1 gives the mixing probabilities and the associated parametricvalues fork(number of components) = 2,3, and 4. It may be noted thatthe Log likelihood value is smaller fork= 4 (the results fork= 5 , 6 etc.are not better than that fork= 4 and hence are not given here). The fourcomponents Poisson Mixture model is given in table 6.2. It may be notedthat 58% of wards may have higher incidence/relative risk and the remainingwards have lesser/lower incidence for the Cancer disease. We computed theposterior probability for each component for each ward (see table 6.3). Eachward is assigned to a particular component so that the posterior probability islarger. These results are also given in table 6.3 Finally we present Choroplethmaps based on those results

Algorithm

Data Sources

Poisson Model

We have analysed the Cancer data of patients in 155 wards of Chennai Cor-poration by the above described method. As preliminary analyses, we havecreated the Choropleth maps for Observed counts, Population of wards, ex-pected counts for patients and SMR's.The Choropleth map for the observed counts Figure 5.2 does not show anypattern. But the Choropleth map for the expected counts Figure: 5.4 indi-cate that the inner regions of the Chennai Corporations have lower expectedcounts and the regions along the border have larger counts of patients. As ameasure of spatial heterogeneity we have computed PSH= 0:7108:Hence ofthe total spatial random variation, nearly 71% is due to spatial heterogene-ity and the remaining 28:92% is due to Poisson variation. Thus the spatialvariation is present in the data.The Choropleth map for Empirical Bayes smoothed rates Figure 5.5 re-veals that only 13 sub regions have high risk values. The wards with numbers53, 64, 67, 70, 78, 93, 100, 103, 110, 117, 122, 147 and 151 have high riskvalues. Though this information could be used by the health managers toconcentrate their work on these regions, one can look for additional covariatesin these regions for further study

Empirical Bayesian Smoothing

Incidence Rate and SMR

Spatial Analysis of Cancer PatientCount Data

Total haemocyte count assay

Spodoptera litura

Insecticidal activity

Inhibition of haemocytes spreading behavior

. Inhibition of haemocytes aggregation behavior

Haemolymph protein profiling

Total haemocyte count (THC)

Haemolymph collection

Asparate (AAT) and Alanine aminotransferase (ALT)

Insect collection

Venomous saliva optimization

Starvation and collection method

Insects Collection

Salivary gland-Histology

Salivary gland-Morphology and Anatomy

Maxillary stylet

Mandibular stylet

Head

Gross morphology and histology of salivary gland

Morphometry of head and stylet

Head and stylet preparation

Insect collection and maintenance

GROSS MORPHOLOGY OF THE REDUVIID HEAD AND SALIVARY GLAND COMPLEX

SDS -PAGE for serum protein analysis

Succinic dehydrogenase

Acid Phosphatase

Protein content

Characteristic features ofLabeo rohita

Survey of lakes to check their status of pollution

Growth maximum values

Rates of growth (OD678/day

Growth

Experimental Set up

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Mechanical composition

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Temperature Gradient Tunnels (TGT)

Free Air Carbon dioxide Enrichment (FACE)Facility

Site

Phenological traits and plant height

Analysis of variance and differences among wheat varieties released in different years in India

Estimation of total N% of wheat grainsand straw

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Normalized difference vegetation index (NDVI)

Spike length (cm)

Last node to spike length(cm)

Peduncle length(cm)

HarvestIndex

Grain yield per plot (g)

Biological Yield(g)

1000 Kernel weight(mg)

Number of grains per spike

Number of productive tillers per meter

Plant height (cm)

Days to physiological maturity

Days to heading

Field observations

Inoculum concentration

STUDY AREA

Tested individually

T cell frequencies(Post vaccination response)

Decreased CD3 ζ chain expression on CD8 T cells in HBsAgpositive newborns

T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.

Clinical characteristics of the subjects

Healthy Controls

1X PBS

Generation of polyclonal antibodies against GFP, APC and β-catenin

Primary antibodies

pGEX6P2-BPN (1-993 a.a)

pET30a-GFP-FL (GFP antigen)

pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)

pET30a-β-catenin-FL (Antigen for -catenin antibody)

GFP-β-cateninC-C (423-634 a.a) (ARM-C)

GFP-β-catenin-C-N (151-423 a.a) (ARM-N)

GFP-β-catenin-C (ARM)

GFP-β-catenin- C (634 -781 a.a)

GFP-β-catenin- a.a

GFP-β-catenin T41A mutant

Constructs made in the study

GFP-β-catenin-FL

passer

Cell culture and drug treatment

Commonly used Buffers/Reagents

IL-2 Bioassay