Note that mentions tagged by “Incorrect” and“InsufficientMetaData” are deemed not legitimate and it is desirable that RDW andRRID-by-RDW not identify them.

Papers containing SCR RRID

Summary and Conclusions

The Use of RRIDs vs Data Citation

corpi

where authors did not report an RRID forthe resource that they used, constituting 37% of all RRID mentions identified by SciBot

RDW recognized mentions of digital resource names, RRIDs or URLs from a total of701110 articles

Nature Research supports the Resource Identification Initiative, with the aim of promoting unique, persistent identification and tracking of key biological resources, including antibodies, cell lines, model organisms and tools.   We encourage authors to include unique identifiers provided by the Resource Identification Portal, (RRIDs; for example, Antibody: RRID:AB_2140114; Organism: RRID:MGI_MGI:3840442), in the manuscript.  More information on how to include listed RRIDs or generate new RRIDs can be found on the Resource Identification Portal.

AB_2231063

Research resources used in this publication

We used a mouse line expressing EGFP under the control of the 5Htr3aR-promoter (5HT3aR-BACEGFP ) provided by the GENSAT project at Rockefeller University.

AM251

NGF

These differences between lots and the possibility that the lot that we used was subject to precipitation during the experiment raises the possibility that the differences between our results and those of Erschbamer et al. are due to differences between lots of PD168393. For example, it is possible that the compound precipitated somewhat in our experiments, perhaps clogging the intra-spinal cannulae whereas the lot used by Erschbamer did not precipitate. Erschbamer et al. do not indicate the lot number for the PD168393 that they used in their paper, but subsequent discussions reveal that the lot number for the original study was B61311. According to the manufacturer, the differences in the numbering indicate that lot B61311 and D00069257 were synthesized at different times, whereas lot D00069257 and D00078517 were synthesized at the same time but packaged at different times. Unfortunately, the company has been unable or unwilling to provide material from previous batches, so it has not been possible to carry out direct head-to-head comparisons between different lots.

Publishers are also getting involved: around a dozen journals this year began asking their authors to use unique identifiers for their reagents as part of a push by the Resource Identification Initiative.

Many large data providers in life sciences did not adapt LSIDs, probably because it meant too many changes to their architecture. Thus the exposure, knowledge and skills around LSIDs did not propagate to the masses.

RRID:AB_87705

RRID: AB_2301751;

gainst RelA (polyclonal C-20

It was transferred to Boehringer-Mannheim as Clone 12H11, resold to Roche and finally bought by Chemicon, and it is now sold as MAB3026.

It is clear from the use of ES2 and RMG-II cell lines that the Atlas Antibodies ARID1A antibody is specific for ARID1A in both Western blots and formalin-fixed paraffin embedded preparations of human origin and, coupled with the literature evidence, that it is validated in human tissue.

A No Primary antibody control (NPA) showed no staining in the epithelial or nuclear compartment (Figure 3B; Dataset e).

There was no cytoplasmic or extracellular stromal background staining present and the antibody titrated successfully losing the intensity of staining, as expected

Control slides, omitting the primary antibody, were negative except for the ER2 condition in the RMG-II cell pellet where a weak cytoplasmic background could be seen (Figure 2; Dataset d). Thus there was minimal background inherent in the staining procedure. It was therefore determined that the antibody showed specificity for formalin-fixed paraffin embedded tissues and could be run on murine tissue.

It could be demonstrated that the HPA ARID1A antibody showed positive expression in ES-2 cell lines at the expected size of 270 kDa and no staining for RMG-II.

anti-MLH1 (550838, clone G168-15, dilution 1:50; BD Pharmingen, Franklin Lakes, NJ, USA

goat polyclonal anti-actin (sc-1616

mouse p-p44/42

mouse SphK1

NF-κB p65 subunit from Abcam (ab7970

p65 from Chemicon (MAB 3026

anti-cytokeratin 19 (SC-6278)

anti-p50 (SC-7178)

monoclonal pSer-536 clone 93H1

NLS-RelA (monoclonal MAB 3026 clone 12H11, 1∶250; Millipore, Darmstadt, Germany