Minipreparation of plasmid DNA from transformed co

Screening of recombinant E. coli clone

Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)

Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)

Ligation reactions

Dephosphorylation of vector

Restriction digestion

Ligation of eluted PCR product in pBSK vector

Cloning of gel eluted PCR produc

Gel elution of PCR Produ

Scoring of amplification data points and construction of a den

Analysis of PCR amplicons using agarose gel electrophoresis

Cycling condition

PCR reaction set up

Basic requirements for PC

PCR amplification of Dof domain and Dof genes from different

Primer designing for PCR amplification of Dof domain and D

Qualitative analysis of DNA by agarose gel electrophoresi

Spectrophotometric quantification of genomic DNA

DNA purification

DNA extraction procedure

Isolation of genomic DNA by CTAB method

Glasswares, plasticwares and equipments

Immunological Responses (DTH, mitogenic and Leishmania-specific cellular responses)

Formaldehyde gel electrophoresis

LTT assay

2 Anim

Nitrite production in macrophages of hamsters

Animals

rLdTPR was cloned, overexpressed, purified and antibody rais

Overexpression and Purification of recombinant protein (rLdTP

Clone confirmation

Colony PCR

Transformation

PCR amplification

2D QSAR

Active site analysis

Inhibitors Dataset

Assay for Lipid peroxidase(LPO)

Cytomorphological analysis

Growth kinetic studies

Reducing power assay

TEM-EDAX analysis

Qualitative analysis of compounds

The table 6.1 gives the mixing probabilities and the associated parametricvalues fork(number of components) = 2,3, and 4. It may be noted thatthe Log likelihood value is smaller fork= 4 (the results fork= 5 , 6 etc.are not better than that fork= 4 and hence are not given here). The fourcomponents Poisson Mixture model is given in table 6.2. It may be notedthat 58% of wards may have higher incidence/relative risk and the remainingwards have lesser/lower incidence for the Cancer disease. We computed theposterior probability for each component for each ward (see table 6.3). Eachward is assigned to a particular component so that the posterior probability islarger. These results are also given in table 6.3 Finally we present Choroplethmaps based on those results

The Posterior Probability of Mixing Dis-tribution

Algorithm

EM Procedure

Poisson Mixture

Data Sources

Poisson Mixtures Distribution

Poisson Model

Haemolymph protein profile

Helicoverpa armiger

Spodoptera litura

Gut enzyme profile

Haemolymph protein profiling

Lactate dehydrogenase

VS preparation

Utilization of VS by the prey

Influence of prey on VS yield

Quantification of protein of VS yielded in the prey deprivation

Survival (SR) and venom milking rate (VMR

Venomous saliva optimization

Statistical analysi

Quantification of VS for protein

Venomous saliva utilization

Prey type

Starvation and collection method

Venomous saliva optimization

Insects Collection

VENOMOUS SALIVA: OPTIMIZATION AND UTILIZATION

Mandibular stylet

Head and stylet preparation

Micronuclei test

Malic dehydrogenase

Alkaline Phosphatase

Glycogen content

Analysis of Physico-chemical parameters of the water sampled

Increments in biomass:

Residual nutrients

Chlorophyll content in waters:

Growth maximum values

Rates of growth (OD678/day

Growth

Experimental Set up

BIOMASS PRODUCTION: ROLE OF N-P CONCENTRATION AND RATIOS

Soil pH and electrical conductivity (EC)

Experiment 2: Assessing the impacts of elevated temperature and N levels on yield and nutrient uptake in rice

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Experimental Design and Treatments

Temperature Gradient Tunnels (TGT)

Yield and its component traits:

Differences among cultivars for yield, biomass and HI

Kernel hardness

SDS-sedimentation test

Estimation of total N% of wheat grainsand straw

End use Quality parameters

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Laboratory observations

Days to physiological maturity

Static and shaken condition

Screening for proteolytic activity

Vaccination improved the Chemokine receptor CCR1, CCR3, CCR9 and Toll like receptor TLR2, TLR4 and TLR9 expression in HBsAgpositive newborns compared to healthy newborns.

IFN γ production by CD8 T cells upon stimulation with PMA and viral peptides

Pre-vaccination:Lower Chemokine and Toll like receptor expression in HBsAgpositive newborns:

T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.

Sample Collection

1X TBS

Immunofluorescence

Secondary antibodies

Animals

GFP-β-catenin T41A mutant

Transfection of short interfering RNA (siRNA) and plasmid

Mammalian cell lines

Assay of IFN-yand IL-10 using sandwich ELISA

Anti-Stm and anti-E coli /gG subclass assay

Mice

Cellline

Extraction of Tannin

Extraction

Procedure

Standard curve of sugar

Extraction and determination of protein

Standard curve of protein

Reagents

Estimation of protein

Standard curve of ascorbic acid

Fresh and Dry weight

Dilution Formula

Cd (NO3)2

ZnSO4

Preparation of ppm solution

Youman's modified medium

Histopathological studies

Bacterial Cultures

Preparation of ultra competent E. coli

Antibiotics

Monoclonal Antibodies against the Human Glycophorin-

Monoclonal Antibodies against the Human Glycophorin

Isolation of endothelial cells from rat aorta

Procedur

Procedur

Procedure

Procedure

Reagent

Total antioxidant activit

Procedur

Catalyst Preparation

Catalyst Preparation

Catalyst Preparation

Single Cell Test

Electrochemical Measuremen

Physical Characterization

Catalyst Preparation

Material

ELECTROGENERATION OF SEAWATER ON ALCOHOL OXIDATION IN AN NON-MEMBRANE POWER SYSTEM

Total dissolved solids

High Performance Liquid Chromatography (HPLC) analysis

High Performance Thin Layer Chromatography (HPTLC) analysis

. Thin Layer Chromatography (TLC) analysis

Preliminary phytochemical screening (Ali, 1998; Evans, 2002)

Microbial Contamina

Foaming Index

pH values

Ash values

Extractive value

. Physico-chemical standardization

Standardization of ethanolic extract of E.ribes (WHO, 1998; IndianPharmacopoeia, 1996)

Estimation of Stevioside

Estimation of Steviol

Estimation of total phenols

Somatotype Categories:

somatotype Categories:

somatotype Categories:

Range of Component Ratings:

Somatocharts:

Mean Somatotypes:

SOMATOTYPE DISTRIBUTION OF RURAL SAMPLE

Somatocharts:

Balanced mesomorph:

Overnight grown primary culture of E. coli cells (1 % v/v final concentration) was inoculated into 1 litre of LB media containing antibiotics. Culture was incubated at 37 oc at 200 rpm. Growth was monitored by measuring absorbance of E. coli broth at 600 nm. Culture was induced by adding 1 mM IPTG at an OD of 0.6 and was harvested after 4 hrs of induction. Samples were taken on an hourly basis after induction to check the kinetics of protein expression. Un-induced and induced E. coli cells were analyzed by SDS-PAGE to check the expression of recombinant protein.

Growth and expression of recombinant proteins in E. coli cells

The [3-PMB and a-PMB chains were eluted with a linear gradient of 500 ml each of 0.01 M potassium phosphate buffer (pH 6.5) and 0.015 M potassium phosphate buffer (pH 8.5) at a flow rate of 50 ml/hour. The chains were separately concentrated using Centriprep concentrators (Amicon) and stored in liquid nitrogen till further use

The heme bound a and ~ subunits were obtained as described by Bucci (1981 ). Briefly, hemoglobin was reacted with PMB in an eight fold molar excess (8 moles of PMB per mole of hemoglobin). The reaction mixture was dialyzed extensively against 0.01 M potassium phosphate buffer (pH 6.5) and then loaded onto a CM52 column (30cm x !Scm) that was pre-equilibrated with the same buffer.

Separation of the a and f3 subunits of hemoglobin

Paduans

Riding over the hills, and eating their fill,7 lying a little too long; these things are, perhaps, enough to explain what happened. How­ever, that may be: they woke suddenly from a sleep they had never meant to take. The standing stone was cold, and it cast a long pale shadow. The sun was gleaming through the mist; north, south, and east, the fog was thick, cold and white. The air was silent, heavy and chill.The hobbits8 sprang to their feet in alarm, and ran to the western rim. They found that they were upon an island in the fog. Even as they looked out in dismay towards the setting sun, it sank before their eyes into a white sea, and a cold grey shadow sprang up in the East behind. The fog rolled up to the walls and rose above them, and as it mounted it bent over their heads until it became a roof. They felt as if a trap was closing about them. They packed up as quickly as their chilled fingers would work.Soon they were leading their ponies in single file9 over the rim and down the long northward slope of the hill, down into a foggy sea. As they went down the mist became colder and damper, and their hair hung lank and dripping on their foreheads. When they reached the bottom it was so cold that they halted and got out cloaks and hoods, which soon became bedewed with grey drops. Then, mounting their ponies, they went slowly on again. To prevent their getting separated and wandering in different directions they went in file, with Frodo leading. Suddenly Frodo saw a hopeful sign. On either side ahead a darkness began to loom through the mist; and he guessed that they were at last approaching the gap in the hills. 'Come on! Follow me!' he called back over his shoulder, and he hurried forward. His pony reared, and he fell off. When he looked back he found that he was alone: the others had not fol­lowed him.

Riding over the hills, and eating their fill,7 lying a little too long; these things are, perhaps, enough to explain what happened. How­ever, that may be: they woke suddenly from a sleep they had never meant to take. The standing stone was cold, and it cast a long pale shadow. The sun was gleaming through the mist; north, south, and east, the fog was thick, cold and white. The air was silent, heavy and chill.The hobbits8 sprang to their feet in alarm, and ran to the western rim. They found that they were upon an island in the fog. Even as they looked out in dismay towards the setting sun, it sank before their eyes into a white sea, and a cold grey shadow sprang up in the East behind. The fog rolled up to the walls and rose above them, and as it mounted it bent over their heads until it became a roof. They felt as if a trap was closing about them. They packed up as quickly as their chilled fingers would work.Soon they were leading their ponies in single file9 over the rim and down the long northward slope of the hill, down into a foggy sea. As they went down the mist became colder and damper, and their hair hung lank and dripping on their foreheads. When they reached the bottom it was so cold that they halted and got out cloaks and hoods, which soon became bedewed with grey drops. Then, mounting their ponies, they went slowly on again. To prevent their getting separated and wandering in different directions they went in file, with Frodo leading. Suddenly Frodo saw a hopeful sign. On either side ahead a darkness began to loom through the mist; and he guessed that they were at last approaching the gap in the hills. 'Come on! Follow me!' he called back over his shoulder, and he hurried forward. His pony reared, and he fell off. When he looked back he found that he was alone: the others had not fol­lowed him.

The clear cell-free supernatants were used as the source of crude recombinant xylanase.

Quantitative screening for determination of xylanase in shake flask

2 mL of an overnight culture of E. coli cells was inoculated into 100 mL LB medium and incubated with vigorous shaking at 30 °C until A600 of 0.8 was reached. •Cells were collected in 50 mL plastic (Falcon) tubes, cooled for 15 min on ice and centrifuged in a pre-cooled centrifuge (4,000 rpm for 10 min at 4 °C). •The pellet was suspended in 20 mL of ice-cold 50 mM CaCl2-15% glycerol solution, maintained on ice for 15 min and centrifuged again at 4,000 rpm for 10 min at 4 °C. •Pellet was resuspended in 2 mL of ice-cold 50 mM CaCl2-15 % glycerol solution, kept on ice for 30 min and aliquoted in 400 μL in microcentrifuge tubes. These were stored at -80 °C until required.

Preparation of calcium-competent cells