16 Matching Annotations
  1. Dec 2023
      • for: climate crisis - multiple dimensions, polycrisis - multiple dimensions, climate crisis - good references, polycrisis - good references, polycrisis - comprehensive map, power to the people, climate change - politics, climate crisis - politics

      • comment / summary

        • The content on this website may be what some call "doomers" that support a narrative of unavoidable catastrophe and civilization collapse
        • The author does an excellent job of drawing together many scientifically validated research papers and news media stories on various crisis and integrates them together to support his narrative.
        • As the author states, it is still incomplete but it is comprehensive and detailed enough to use as a starting foundation to build a complex polycrisis map upon. becaues it shows the complexities of the interwoven nexus of problems we face and the massive network of feedbacks between them that makes solving any one of them alone in isolation an impossibility
        • The Cascade Institute focuses on social tipping points, complexity and polycrisis. We could synthesis a number of tools to map out and reveal effective mitigation strategies including:
          • Cascade Institute tools
          • Social tipping point tools
          • SRG mapping tool along with Indyweb / Indranet
          • Culture hacking tools
          • SIMPOL strategy
          • Downscaled Earth System Boundary tools
          • SRG Deep Humanity BEing journey tools
          • James Hansen's recommendation that the biggest leverage point is new form of governance
            • We need to rapidly emerge a new global third political party that does not take money from special interest groups
          • Progressive International comes to the same conclusion as James Hansen, that the key leverage point for rapid whole system change is radically new governance that puts power back to the hands of the people - power to the people
          • SONEC's
          • Indyweb's people-centered, interpersonal methodology is a perfect match for SONEC circle-within-circles fractal structure
            • mention to @Gyuri
            • I've seen this circle-within-circle fractal, holonic group idea with Tim's software as well as Roberto's
        • Feebate from local governance groups (from another Doomer site - Arctic Emergency)
        • What the author's narrative shows is
          • how precarious our situation is
          • how many trends are getting far worse in the immediate future
          • how we are already undercapacitated to deal with existing crisis so how will we deal with new ones that are exponentially worse?
          • all these crisis will impact our supply chains. Why are these important? Our reliance on technology is dangerous and makes us very vulnerable
          • Think of your laptop, cellphone or other electronic device that relies on a vast, complex and globally operational internet. Imagine that tidal surges wipes out the globally critical data centers located in New York. Or imagine electronic factories in China and Taiwan are wiped out due to extreme weather. How will you get or fix a broken piece of electronic equipment? We rely on each millions of specialized jobs all working smoothly in order for our laptop to continue working and communicating with each other.
      • epiphany

      • recommendation for new Indyweb / Indranet tools
        • independent time and date stamp tool for every online, virtual sentence we write so we recognize in a long composition when we inserted a new idea
        • ability to trace rapid trains of thought to reveal how new insights emerge from within our consciousness
      • While writing this, I just recalled that we should have a way to time and date stamp every single virtual online action, like in this annotation because recall happens so nonlinearly and we won't have a hope to trace and trailmark without it. Hypothesis doesn't have time and date stamps of every sentence available to the user. So we don't know what nonlinear memory recall led to a specific sentence in an annotation. We need some independent Indyweb / Indranet tool that will do this universally. Trains of thoughts are so fragile we can forget the quick cascades very easily.
  2. Nov 2023
  3. Jul 2023
    1. approaches, which typically encapsulate bacteria in hydrogels, have produced deployable optical sensors for explosives14, heavy metals15 and chemical inducers16,17
    1. Translocation to a cell’s surface utilizes a signal peptide (for inner membrane translocation) and AIDAc as an outer membrane autotransporter pore
    1. AHL communication modules with functional devices built from the biological components of the las18, tra18, rpa18, rhl19, cin19 and esa20 quorum-sensing systems
  4. Jun 2023
    1. programming environmental microbes have been applied to a wide range of organisms. Some examples of newly-programmable microbes include

      good citations of engineering non-model organisms

    1. To date, serine integrases have not been used extensively in plant systems

      although they have been shown to work in principle in Arabidopsis23, Nicotiana benthamiana2421, barley25 and wheat26

  5. Mar 2023
    1. A survey of 230 diverse bacterial and archaeal genomes found evidence of DNA methylation in 93% of genomes, with a diverse array of methylated motifs (834 distinct motifs; average of three motifs per organism)
    1. conjugative plasmids have broad-host ranges23, are resistant to restriction-modification systems24, are easy to engineer with large coding capacities25, and do not require a cellular receptor26 that would provide a facile mechanism for bacterial resistance.
    1. Bacterial cells are typically one thousandth the volume of mammalian cells, which places them near the edge of instrument detection. At this size it can be challenging to differentiate viable cells from debris of similar size
    1. Detailed descriptions, assumptions, limitations and test cases of many popular statistical methods for ecological research can be found in the GUSTAME server (Buttigieg and Ramette, 2014), and in the review by Paliy and Shankar (2016).
    2. condensing the information into two- or three-dimensional spaces. A very good overview of techniques to achieve this was written by Paliy and Shankar (2016).
    1. There are several widely used tool collections, e.g., QIIME 2 [13], mothur [14], usearch [15], and vsearch [16], and 1-stop pipelines, e.g., LotuS [17], with new approaches continually being developed, e.g., OCToPUS [18] and PEMA [19]
    1. Recently, redox-responsive biomolecules such as phenazines have been used in several electrochemical strategies to interrogate a range of biological activities30,31 and to control gene expression in living cells32,33, where the redox status of the biomolecules could be measured or manipulated by application of electronic potentials
  6. Jan 2021
    1. cellular data recorders offer the capacity to measure biologically relevant signals15,16,17,18,19 in places that are otherwise difficult to access, such as inside the body20,21, and over time22